Electron microscopy: The key to resolve RNA viruses replication organelles.

IF 2.6 2区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Microbiology Pub Date : 2024-04-01 Epub Date: 2023-09-30 DOI:10.1111/mmi.15173

Debora Stelitano, Mirko Cortese

引用次数: 0

Abstract

Positive-sense single-stranded RNA viruses significantly reshape intracellular membranes to generate viral replication organelles that form a controlled niche in which nucleic acids, enzymes, and cofactors accumulate to assure an efficient replication of the viral genome. In recent years, advancements in electron microscopy (EM) techniques have enabled imaging of these viral factories in a near-native state providing significantly higher molecular details that have led to progress in our general understanding of virus biology. In this review, we describe the contribution of the cutting-edge EM approaches to the current knowledge of replication organelles biogenesis, structure, and functions.

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电子显微镜：解析RNA病毒复制细胞器的关键。

正向单链RNA病毒显著重塑细胞内膜，产生病毒复制细胞器，形成一个可控的小生境，核酸、酶和辅因子在其中积累，以确保病毒基因组的有效复制。近年来，电子显微镜（EM）技术的进步使这些病毒工厂能够在接近天然状态下成像，提供了更高的分子细节，这使我们对病毒生物学的总体理解取得了进展。在这篇综述中，我们描述了尖端EM方法对复制细胞器生物发生、结构和功能的当前知识的贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Microbiology 生物-生化与分子生物学

CiteScore

7.20

自引率

5.60%

发文量

132

审稿时长

1.7 months

期刊介绍： Molecular Microbiology, the leading primary journal in the microbial sciences, publishes molecular studies of Bacteria, Archaea, eukaryotic microorganisms, and their viruses. Research papers should lead to a deeper understanding of the molecular principles underlying basic physiological processes or mechanisms. Appropriate topics include gene expression and regulation, pathogenicity and virulence, physiology and metabolism, synthesis of macromolecules (proteins, nucleic acids, lipids, polysaccharides, etc), cell biology and subcellular organization, membrane biogenesis and function, traffic and transport, cell-cell communication and signalling pathways, evolution and gene transfer. Articles focused on host responses (cellular or immunological) to pathogens or on microbial ecology should be directed to our sister journals Cellular Microbiology and Environmental Microbiology, respectively.