Colonization dynamics of subgingival microbiota in recently installed dental implants compared to healthy teeth in the same individual: a 6-month prospective observational study.
Carina Maciel Silva-Boghossian, Pablo Torquilho Duarte, Denise Gome da Silva, Talita Gomes Baêta Lourenço, Ana Paula Vieira Colombo
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引用次数: 0
Abstract
Objectives: To evaluate the colonization dynamics of subgingival microbiota established over six months around newly installed dental implants in periodontally healthy individuals, compared with their corresponding teeth.
Methodology: Seventeen healthy individuals assigned to receive single dental implants participated in the study. Subgingival biofilm was sampled from all implant sites and contralateral/ antagonist teeth on days 7, 30, 90, and 180 after implant installation. Microbiological analysis was performed using the Checkerboard DNA-DNA hybridization technique for detection of classical oral taxa and non-oral microorganisms. Significant differences were estimated by Mann-Whitney and Friedman tests, while associations between implants/teeth and target species levels were assessed by linear regression analysis (LRA). Significance level was set at 5%.
Results: Levels of some species were significantly higher in teeth compared to implants, respectively, at day 7 ( V.parvula , 6 × 10 5 vs 3 × 105 ; Milleri streptococci , 2 × 10 6 vs 6 × 10 5 ; Capnocytophaga spp., 2 × 10 6 vs 9 × 10 5 ; E.corrodens , 2 × 10 6 vs 5 × 10 5 ; N. mucosa , 2 × 10 6 vs 5 × 10 5 ; S.noxia , 2 × 10 6 vs 3 × 10 5 ; T.socranskii , 2 × 10 6 vs 5 × 10 5 ; H.alvei , 4 × 10 5 vs 2 × 10 5 ; and Neisseria spp., 6 × 10 5 vs 4 × 10 4 ), day 30 ( V.parvula , 5 × 10 5 vs 10 5 ; Capnocytophaga spp., 1.3 × 10 6 vs 6.8 × 10 4 ; F.periodonticum , 2 × 10 6 vs 10 6 ; S.noxia , 6 × 10 5 vs 2 × 10 5 ; H.alvei , 8 × 10 5 vs 9 × 10 4 ; and Neisseria spp., 2 × 10 5 vs 10 6 ), day 120 ( V.parvula , 8 × 10 5 vs 3 × 10 5 ; S.noxia , 2 × 10 6 vs 0; and T.socranskii , 3 × 10 5 vs 8 × 10 4 ), and day 180 ( S.enterica subsp. enterica serovar Typhi, 8 × 10 6 vs 2 × 10 6 ) (p<0.05). Implants showed significant increases over time in the levels of F.nucleatum , Gemella spp., H.pylori , P.micra , S.aureus , S.liquefaciens , and T.forsythia (p<0.05). LRA found that dental implants were negatively correlated with high levels of S. noxia and V. parvula (β=-0.5 to -0.3; p<0.05).
Conclusions: Early submucosal microbiota is diverse and only a few species differ between teeth and implants in the same individual. Only 7 days after implant installation, a rich microbiota can be found in the peri-implant site. After six months of evaluation, teeth and implants show similar prevalence and levels of the target species, including known and new periodontopathic species.
目的：与相应的牙齿相比，评估牙周健康个体在新安装的牙种植体周围六个月内建立的龈下微生物群的定殖动力学。方法：17名被分配接受单一种植牙的健康个体参与了这项研究。在植入物安装后的第7、30、90和180天，从所有植入部位和对侧/对侧牙齿中采集龈下生物膜。使用棋盘DNA-DNA杂交技术进行微生物分析，以检测经典口腔微生物和非口腔微生物。Mann-Whitney和Friedman检验估计了显著差异，而植入物/牙齿和目标物种水平之间的相关性则通过线性回归分析（LRA）进行了评估。显著性水平设定为5%。结果：某些物种在牙齿中的水平分别显著高于种植体，在第7天（细小病毒，6×105 vs 3×105；Milleri链球菌，2×106 vs 6×105；Capnocytophaga spp.，2×10 6 vs 9×105；腐蚀性大肠杆菌，2×106 vs 5×105；粘膜N.much，2×106vs 5×10 5；诺氏菌，2×10-6 vs 3×105:索氏T.socranskii，2×1006 vs 5×105:H.alvei，4×105vs 2×105:奈瑟菌spp.，6×105vs 4×104），第30天（V.parvula，5×105 vs 10 5；Capnocytophaga spp.，1.3×10 6 vs 6.8×10 4；F.periodontum，2×10 6 vs10 6；S.noxia，6×10 5 vs 2×10 5；H.alvei，8×10 5 vs9×10 4和Neisseria spp.，2×5 vs 10 6），第120天（V.parvula，8×105 vs 3×10 5，S.noxias，2×106 vs 0；和T.socranskii，3×105 vs 8×10 4），和第180天（伤寒肠炎亚种，8×106 vs 2×106）（p结论：早期粘膜下微生物群多种多样，同一个体的牙齿和种植体之间只有少数物种不同。种植体安装后仅7天，就可以在种植体周围发现丰富的微生物群。经过6个月的评估，牙齿和种植物显示出相似的患病率和目标物种水平，包括已知和新的牙周病物种。）。
期刊介绍：
The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.
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