Thimet oligopeptidase (THOP 1) distribution in cane toad (Bufo Marinus, Linnaeus, 1758) brain

IF 2.7 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of chemical neuroanatomy Pub Date : 2023-09-30 DOI:10.1016/j.jchemneu.2023.102345

Diogo M.L.P. Cavalcanti , Tiago S. Teófilo , Tayline D. Rodrigues , Tayssa N.S. Barbosa , José D. Fontenele-Neto

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引用次数: 0

Abstract

Thimet oligopeptides (THOP 1) is a metal-dependent peptidase involved in the metabolism of neuropeptides and the presentation of peptides via MHC-1. It has been shown to play a role in the regulation of protein-protein interactions and the metabolism of intracellular peptides. THOP 1 is associated with important biological processes such as metabolism and neurodegenerative diseases, among others. The objective of this study is to elucidate the distribution of THOP 1 in the Bufo marinus brain. The analysis of THOP 1 amino acid sequences indicates that they have been conserved throughout evolution, with significant homology observed across various phyla. When comparing amphibians with other species, more than 70% identity can be identified. Immunohistochemistry analysis of the toad's brain has demonstrated that the enzyme has a ubiquitous distribution, consistent with previous findings in mammals. THOP 1 can be found in important areas of the brain, such as bulb, thalamic nuclei, striatum, hypothalamus, and among others. Nonetheless, THOP 1 is consistently localized within the nucleus, a pattern also observed in the rat brain. Therefore, based on these results, the toad appears to be an excellent model for studying the general biology of THOP 1, given the substantial homology of this enzyme with mammals and its similarity in distribution within the brain.

查看原文本刊更多论文

Thimet寡肽酶（THOP1）在蟾蜍（Bufo Marinus，Linnaeus，1758）脑中的分布。

Thimet寡肽（THOP1）是一种金属依赖性肽酶，参与神经肽的代谢和通过MHC-1呈递肽。它已被证明在蛋白质-蛋白质相互作用和细胞内肽代谢的调节中发挥作用。THOP 1与代谢和神经退行性疾病等重要的生物学过程有关。本研究的目的是阐明THOP1在蟾蜍脑中的分布。对THOP 1氨基酸序列的分析表明，它们在整个进化过程中都是保守的，在不同的门中观察到显著的同源性。当将两栖动物与其他物种进行比较时，可以确定70%以上的身份。蟾蜍大脑的免疫组织化学分析表明，这种酶具有普遍的分布，与之前在哺乳动物中的发现一致。THOP 1可以在大脑的重要区域发现，如灯泡、丘脑核、纹状体、下丘脑等。尽管如此，THOP 1始终定位在细胞核内，这一模式也在大鼠大脑中观察到。因此，基于这些结果，蟾蜍似乎是研究THOP 1一般生物学的优秀模型，因为这种酶与哺乳动物具有显著的同源性，并且在大脑中的分布相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chemical neuroanatomy 医学-神经科学

CiteScore

4.50

自引率

3.60%

发文量

审稿时长

62 days

期刊介绍： The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.