Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing.

IF 10.8 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Pa Reum Lee, Jihoon Kim, Heather Lynn Rossi, Sena Chung, Seung Yub Han, Junhyong Kim, Seog Bae Oh
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Abstract

Dental primary afferent (DPA) neurons and proprioceptive mesencephalic trigeminal nucleus (MTN) neurons, located in the trigeminal ganglion and the brainstem, respectively, are essential for controlling masticatory functions. Despite extensive transcriptomic studies on various somatosensory neurons, there is still a lack of knowledge about the molecular identities of these populations due to technical challenges in their circuit-validated isolation. Here, we employed high-depth single-cell RNA sequencing (scRNA-seq) in combination with retrograde tracing in mice to identify intrinsic transcriptional features of DPA and MTN neurons. Our transcriptome analysis revealed five major types of DPA neurons with cell type-specific gene enrichment, some of which exhibit unique mechano-nociceptive properties capable of transmitting nociception in response to innocuous mechanical stimuli in the teeth. Furthermore, we discovered cellular heterogeneity within MTN neurons that potentially contribute to their responsiveness to mechanical stretch in the masseter muscle spindles. Additionally, DPA and MTN neurons represented sensory compartments with distinct molecular profiles characterized by various ion channels, receptors, neuropeptides, and mechanoreceptors. Together, our study provides new biological insights regarding the highly specialized mechanosensory functions of DPA and MTN neurons in pain and proprioception.

Abstract Image

使用单细胞RNA测序的牙齿感觉和本体感觉三叉神经神经元的转录谱。
牙齿初级传入(DPA)神经元和本体感觉中脑三叉神经核(MTN)神经元分别位于三叉神经节和脑干,对控制咀嚼功能至关重要。尽管对各种体感神经元进行了广泛的转录组学研究,但由于电路验证分离的技术挑战,对这些群体的分子身份仍然缺乏了解。在这里,我们在小鼠中采用高深度单细胞RNA测序(scRNA-seq)结合逆行追踪来鉴定DPA和MTN神经元的内在转录特征。我们的转录组分析揭示了具有细胞类型特异性基因富集的五种主要类型的DPA神经元,其中一些表现出独特的机械伤害感受特性,能够对牙齿中无害的机械刺激进行传递伤害感受。此外,我们发现MTN神经元内的细胞异质性可能有助于它们对咬肌纺锤体机械拉伸的反应。此外,DPA和MTN神经元代表了具有不同分子特征的感觉区室,其特征是各种离子通道、受体、神经肽和机械感受器。总之,我们的研究为DPA和MTN神经元在疼痛和本体感觉中的高度专业化机械感觉功能提供了新的生物学见解。
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来源期刊
International Journal of Oral Science
International Journal of Oral Science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
31.80
自引率
1.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: The International Journal of Oral Science covers various aspects of oral science and interdisciplinary fields, encompassing basic, applied, and clinical research. Topics include, but are not limited to: Oral microbiology Oral and maxillofacial oncology Cariology Oral inflammation and infection Dental stem cells and regenerative medicine Craniofacial surgery Dental material Oral biomechanics Oral, dental, and maxillofacial genetic and developmental diseases Craniofacial bone research Craniofacial-related biomaterials Temporomandibular joint disorder and osteoarthritis The journal publishes peer-reviewed Articles presenting new research results and Review Articles offering concise summaries of specific areas in oral science.
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