Expression deregulation of genes related to DNA repair and lead toxicity in occupationally exposed industrial workers.

IF 2.6 3区医学 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

International Archives of Occupational and Environmental Health Pub Date : 2023-12-01 Epub Date: 2023-10-07 DOI:10.1007/s00420-023-02012-4

Zertashia Akram, Ishrat Mahjabeen, Mariam Batool, Sana Kanwal, Fatima Nawaz, Mahmood Akhtar Kayani, Muhammad Rizwan

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引用次数: 0

Abstract

Objective: Globally millions of people working in various industries and are exposed to different toxins which may affect their genetic stability and DNA integrity. Present study was designed to estimate the expression variation of genes related to DNA repair (XRCC1, PARP1) and lead toxicity (ALAD) in exposed industrial workers.

Methods: About 200 blood samples were collected from workers of brick kiln, welding, furniture and paint industry (50/industry) along with age and gender matched controls. mRNA expression of genes was measured using RT-PCR. Serum levels of total ROS, POD, TBAR activity was calculated. Blood lead levels were estimated by atomic absorption spectrometer.

Results: Relative expression of XRCC1 and PARP1 gene was significantly (P < 0.001) upregulated, while ALAD gene expression was downregulated in exposed group compared to control. Expression of XRCC1 and PARP1 was increased (P < 0.001) in exposed workers with > 30 year age compared to control with > 30 year age. Same was observed when < 30 year age group of control and exposed was compared. Likewise, XRCC1 and PARP1 expression was increased (P < 0.001) in exposed workers with > 30 year age compared to workers with < 30 year age. Whereas, ALAD gene showed significant (P < 0.01) decrease in > 30 year age workers compared to control of same age and exposed with < 30 year of age. Relative expression of XRCC1 and PARP1 was increased (P < 0.001) in exposed smokers compared to exposed non-smokers and control smokers. Whereas, ALAD gene expression reduced (P < 0.001) significantly in both groups. Blood lead content was higher (P < 0.001) in exposed group compared to control. Strong correlation was observed between XRCC1, PARP1 and ALAD gene versus age, total exposure duration, exposure per day and lead deposition. ROS, TBARS and POD activity was higher (P < 0.01) in exposed group compared to control group.

Conclusion: Present study suggested deregulation of genes related to DNA repair and lead intoxication in exposed group compared to controls. Strong correlation was observed between selected genes and demographic parameters. Present results revealed altered activity of oxidative stress markers which would induce oxidative damage to DNA integrity and limit the function of repair enzymes.

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在职业暴露的工业工人中，与DNA修复和铅毒性相关的基因表达失调。

目标：在全球范围内，数百万在不同行业工作的人暴露在不同的毒素中，这些毒素可能会影响他们的基因稳定性和DNA完整性。本研究旨在评估暴露于环境中的工业工人中与DNA修复（XRCC1，PARP1）和铅毒性（ALAD）相关的基因的表达变化。方法：从砖窑、焊接、家具和油漆行业（50/行业）的工人中采集约200份血液样本，并与年龄和性别匹配的对照组进行比较。用RT-PCR检测基因的mRNA表达。计算血清总ROS、POD、TBAR活性水平。用原子吸收光谱仪测定血铅含量。结果：XRCC1和PARP1基因的相对表达显著高于对照组（P 与对照组相比，30岁 > 30岁。当 30岁的工人相比 30岁工人与同龄对照组的比较结论：本研究表明，与对照组相比，暴露组与DNA修复和铅中毒相关的基因失调。所选基因与人口统计学参数之间存在很强的相关性。目前的结果揭示了氧化应激标记物的活性改变，这将诱导对DNA完整性的氧化损伤并限制修复酶的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Archives of Occupational and Environmental Health 医学-公共卫生、环境卫生与职业卫生

CiteScore

5.60

自引率

6.70%

发文量

127

审稿时长

3 months

期刊介绍： International Archives of Occupational and Environmental Health publishes Editorials, Review Articles, Original Articles, and Letters to the Editor. It welcomes any manuscripts dealing with occupational or ambient environmental problems, with a special interest in research at the interface of occupational health and clinical medicine. The scope ranges from Biological Monitoring to Dermatology, from Fibers and Dust to Human Toxicology, from Nanomaterials and Ultra-fine Dust to Night- and Shift Work, from Psycho-mental Distress and Burnout to Vibrations. A complete list of topics can be found on the right-hand side under For authors and editors. In addition, all papers should be based on present-day standards and relate to: -Clinical and epidemiological studies on morbidity and mortality -Clinical epidemiological studies on the parameters relevant to the estimation of health risks -Human experimental studies on environmental health effects. Animal experiments are only acceptable if relevant to pathogenic aspects. -Methods for studying the topics mentioned above.