Domestic cat hepadnavirus detection in blood and tissue samples of cats with lymphoma.

IF 7.9 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary Quarterly Pub Date : 2023-12-01 Epub Date: 2023-10-09 DOI:10.1080/01652176.2023.2265172

Chutchai Piewbang, Sabrina Wahyu Wardhani, Jedsada Siripoonsub, Sirintra Sirivisoot, Anudep Rungsipipat, Somporn Techangamsuwan

{"title":"Domestic cat hepadnavirus detection in blood and tissue samples of cats with lymphoma.","authors":"Chutchai Piewbang, Sabrina Wahyu Wardhani, Jedsada Siripoonsub, Sirintra Sirivisoot, Anudep Rungsipipat, Somporn Techangamsuwan","doi":"10.1080/01652176.2023.2265172","DOIUrl":null,"url":null,"abstract":"Domestic cat hepadnavirus (DCH), a relative hepatitis B virus (HBV) in human, has been recently identified in cats; however, association of DCH infection with lymphoma in cats is not investigated. To determine the association between DCH infection and feline lymphoma, seven hundred and seventeen cats included 131 cats with lymphoma (68 blood and 63 tumor samples) and 586 (526 blood and 60 lymph node samples) cats without lymphoma. DCH DNA was investigated in blood and formalin-fixed paraffin-embedded (FFPE) tissues by quantitative polymerase chain reaction (qPCR). The FFPE lymphoma tissues were immunohistochemically subtyped, and the localization of DCH in lymphoma sections was investigated using in situ hybridization (ISH). Feline retroviral infection was investigated in the DCH-positive cases. DCH DNA was detected in 16.18% (11/68) (p = 0.002; odds ratio [OR], 5.15; 95% confidence interval [CI], 2.33-11.36) of blood and 9.52% (6/63) (p = 0.028; OR, 13.68; 95% CI, 0.75-248.36) of neoplastic samples obtained from lymphoma cats, whereas only 3.61% (19/526) of blood obtained from non-lymphoma cats was positive for DCH detection. Within the DCH-positive lymphoma, in 3/6 cats, feline leukemia virus was co-detected, and in 6/6 were B-cell lymphoma (p > 0.9; OR, 1.93; 95% CI, 0.09-37.89) and were multicentric form (p = 0.008; OR, 1.327; 95% CI, 0.06-31.18). DCH was found in the CD79-positive pleomorphic cells. Cats with lymphoma were more likely to be positive for DCH than cats without lymphoma, and infection associated with lymphoma development needs further investigations.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":" ","pages":"1-10"},"PeriodicalIF":7.9000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b8/6b/TVEQ_43_2265172.PMC10563604.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Quarterly","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/01652176.2023.2265172","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/10/9 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 1

Abstract

Domestic cat hepadnavirus (DCH), a relative hepatitis B virus (HBV) in human, has been recently identified in cats; however, association of DCH infection with lymphoma in cats is not investigated. To determine the association between DCH infection and feline lymphoma, seven hundred and seventeen cats included 131 cats with lymphoma (68 blood and 63 tumor samples) and 586 (526 blood and 60 lymph node samples) cats without lymphoma. DCH DNA was investigated in blood and formalin-fixed paraffin-embedded (FFPE) tissues by quantitative polymerase chain reaction (qPCR). The FFPE lymphoma tissues were immunohistochemically subtyped, and the localization of DCH in lymphoma sections was investigated using in situ hybridization (ISH). Feline retroviral infection was investigated in the DCH-positive cases. DCH DNA was detected in 16.18% (11/68) (p = 0.002; odds ratio [OR], 5.15; 95% confidence interval [CI], 2.33-11.36) of blood and 9.52% (6/63) (p = 0.028; OR, 13.68; 95% CI, 0.75-248.36) of neoplastic samples obtained from lymphoma cats, whereas only 3.61% (19/526) of blood obtained from non-lymphoma cats was positive for DCH detection. Within the DCH-positive lymphoma, in 3/6 cats, feline leukemia virus was co-detected, and in 6/6 were B-cell lymphoma (p > 0.9; OR, 1.93; 95% CI, 0.09-37.89) and were multicentric form (p = 0.008; OR, 1.327; 95% CI, 0.06-31.18). DCH was found in the CD79-positive pleomorphic cells. Cats with lymphoma were more likely to be positive for DCH than cats without lymphoma, and infection associated with lymphoma development needs further investigations.

Abstract Image

查看原文本刊更多论文

淋巴瘤猫血液和组织样本中家猫肝附件病毒检测。

家猫肝炎病毒（DCH）是一种人类乙型肝炎病毒（HBV），最近在猫身上被发现；然而，猫DCH感染与淋巴瘤的关系尚未得到研究。为了确定DCH感染与猫淋巴瘤之间的关系，七百一十七只猫包括131只患有淋巴瘤的猫（68份血液和63份肿瘤样本）和586只没有淋巴瘤（526份血液和60份淋巴结样本）。采用定量聚合酶链反应（qPCR）方法对血液和福尔马林固定石蜡包埋（FFPE）组织中DCH DNA进行了研究。对FFPE淋巴瘤组织进行免疫组化分型，并用原位杂交（ISH）研究DCH在淋巴瘤切片中的定位。对DCH阳性病例中的猫逆转录病毒感染进行了调查。DCH DNA检出率为16.18%（11/68）（P = 0.002；比值比[OR]，5.15；95%可信区间为2.33-11.36）和9.52%（6/63）（P = 0.028；或13.68；95%CI，0.75-248.36），而从非淋巴瘤猫获得的血液中只有3.61%（19/526）的DCH检测呈阳性。在DCH阳性淋巴瘤中，猫白血病病毒在3/6只猫中共检出，B细胞淋巴瘤在6/6只猫中检出（P＞0.9；OR，1.93；95%CI，0.09-37.89），为多中心型（P = 0.008；或1.327；95%可信区间0.06-31.18）。在CD79阳性的多形性细胞中发现DCH。患有淋巴瘤的猫比没有淋巴瘤的猫更有可能检测出DCH阳性，与淋巴瘤发展相关的感染需要进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary Quarterly VETERINARY SCIENCES-

CiteScore

13.10

自引率

1.60%

发文量

审稿时长

>24 weeks

期刊介绍： Veterinary Quarterly is an international open access journal which publishes high quality review articles and original research in the field of veterinary science and animal diseases. The journal publishes research on a range of different animal species and topics including: - Economically important species such as domesticated and non-domesticated farm animals, including avian and poultry diseases; - Companion animals (dogs, cats, horses, pocket pets and exotics); - Wildlife species; - Infectious diseases; - Diagnosis; - Treatment including pharmacology and vaccination