Siderophore-producing Pantoea ferrattrahens sp. nov. isolated from a clinical specimen and Pantoea ferramans sp. nov. isolated from soil at the bottom of a pond

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2023-09-22 DOI:10.1111/1348-0421.13097

Ryo Kutsuna, Tohru Miyoshi-Akiyama, Yuki Muramatsu, Moriyuki Hamada, Junko Tomida, Ken Kikuchi, Yoshiaki Kawamura

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Abstract

Two Gram-negative facultative anaerobes were isolated from a sepsis patient with pancreatic cancer (strain PAGU 2156^T) and soil at the bottom of a pond (strain PAGU 2198^T), respectively. These two strains formed haloes around the colonies on chrome azurol S agar plates, indicating the production of siderophores. Two isolates assigned to the genus Pantoea based on the 16S rRNA gene were differentiated from established species by using polymorphic taxonomies. Phylogenetic analysis using four housekeeping genes (gyrB, rpoB, atpD, and infB) showed that strain PAGU 2156^T is closely related to Pantoea cypripedii LMG 2657^T (89.9%) or Pantoea septica LMG 5345^T (95.7%). Meanwhile, strain PAGU 2198^T formed a single clade with Pantoea rodasii DSM 26611^T (93.6%) and Pantoea rwandensis DSM 105076^T (93.3%). The average nucleotide identity values obtained from the draft genome assembly showed ≤90.2% between strain PAGU 2156^T and closely related species and ≤81.5% between strain PAGU 2198^T and closely related species. Based on various phenotypes, biochemical properties, and whole-cell fatty acid composition compared with related species, it was concluded that each strain should be classified as a new species of the genus Pantoea. In this manuscript, Pantoea ferrattrahens sp. nov. and Pantoea ferramans sp. nov. with strain PAGU 2156^T (=NBRC 115930^T = CCUG 76757^T) and strain PAGU 2198^T (=NBRC 114265^T = CCUG 75151^T) are proposed as each type strain.

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从临床标本中分离的产铁载体Pantoea ferrattraens sp. 11和从池塘底部土壤中分离的Pantoea ferramans sp. 11

从1例胰腺癌脓毒症患者和池塘底部土壤中分别分离到2株革兰氏阴性兼性厌氧菌(菌株PAGU 2156T)和2株菌株PAGU 2198T。这两个菌株在蓝铬S琼脂板上菌落周围形成光晕，表明产生了铁载体。采用多态分类方法，将基于16S rRNA基因鉴定的两株Pantoea属分离株与已建立的种进行了区分。利用gyrB、rpoB、atpD和infB 4个内源基因进行系统发育分析，发现菌株PAGU 2156T与Pantoea cypripedii LMG 2657T(89.9%)或Pantoea septica LMG 5345T(95.7%)亲缘关系较近。同时，菌株PAGU 2198T与Pantoea roasii DSM 26611T(93.6%)和Pantoea rwanda DSM 105076T(93.3%)形成一个分支。从基因组组装草图中获得的平均核苷酸同源性值显示，菌株PAGU 2156T与近缘种之间的核苷酸同源性值≤90.2%，菌株PAGU 2198T与近缘种之间的核苷酸同源性值≤81.5%。根据其表型、生化特性和与近缘种的全细胞脂肪酸组成的比较，该菌株应被归类为Pantoea属的一个新种。本文分别选用菌株PAGU 2156T (=NBRC 115930T = CCUG 76757T)和菌株PAGU 2198T (=NBRC 114265T = CCUG 75151T)的Pantoea ferrattraens sp. 11和Pantoea ferramans sp. 11作为菌株类型。

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.