Global and local fat effects on bone mass and quality in obesity.

IF 4.7 2区医学 Q2 CELL & TISSUE ENGINEERING

Bone & Joint Research Pub Date : 2023-09-20 DOI:10.1302/2046-3758.129.BJR-2023-0102.R1

Xin Dai, Beizhong Liu, Qingtao Hou, Qijie Dai, Di Wang, Bo Xie, Yue Sun, Bin Wang

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Abstract

Aims: The aim of this study was to investigate the global and local impact of fat on bone in obesity by using the diet-induced obese (DIO) mouse model.

Methods: In this study, we generated a diet-induced mouse model of obesity to conduct lipidomic and 3D imaging assessments of bone marrow fat, and evaluated the correlated bone adaptation indices and bone mechanical properties.

Results: Our results indicated that bone mass was reduced and bone mechanical properties were impaired in DIO mice. Lipidomic sequencing and bioinformatic analysis identified 373 differential lipids, 176 of which were upregulated and 197 downregulated. Functional enrichment analysis revealed a significant downregulation of the pathways: fat digestion and absorption (ko04975) and lipolysis regulation in adipocytes (ko04923) in DIO mice, leading to local fat accumulation. The use of 3D imaging confirmed the increase in fat accumulation within the bone marrow cavity of obese mice.

Conclusion: Our study sheds light on the intricate interplay between fat and bone, and provides a non-toxic and non-invasive method for measuring marrow adipose tissue.

Abstract Image

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肥胖患者全身和局部脂肪对骨量和质量的影响。

目的：本研究的目的是通过使用饮食诱导肥胖（DIO）小鼠模型来研究脂肪对肥胖小鼠骨骼的全局和局部影响。方法：在本研究中，我们建立了一个饮食诱导的肥胖小鼠模型，对骨髓脂肪进行脂质组学和3D成像评估，并评估相关的骨适应指数和骨力学特性。结果：我们的结果表明，DIO小鼠的骨量减少，骨力学性能受损。脂质组学测序和生物信息学分析鉴定了373种不同的脂质，其中176种上调，197种下调。功能富集分析揭示了以下途径的显著下调：DIO小鼠的脂肪消化和吸收（ko04975）和脂肪细胞中的脂解调节（ko04923），导致局部脂肪积聚。3D成像的使用证实了肥胖小鼠骨髓腔内脂肪积聚的增加。结论：我们的研究揭示了脂肪和骨骼之间复杂的相互作用，并为测量骨髓脂肪组织提供了一种无毒、无创的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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