The influence of first desaturase subfamily genes on fatty acid synthesis, desiccation tolerance and inter-caste nutrient transfer in the termite Coptotermes formosanus

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Molecular Biology Pub Date : 2023-09-26 DOI:10.1111/imb.12877

Danni Xu, Yuxin Tong, Bosheng Chen, Baoling Li, Shengyin Wang, Dayu Zhang

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引用次数: 0

Abstract

Desaturase enzymes play an essential role in the biosynthesis of unsaturated fatty acids (UFAs). In this study, we identified seven “first desaturase” subfamily genes (Cfor-desatA1, Cfor-desatA2-a, Cfor-desatA2-b, Cfor-desatB-a, Cfor-desatB-b, Cfor-desatD and Cfor-desatE) from the Formosan subterranean termite Coptotermes formosanus. These desaturases were highly expressed in the cuticle and fat body of C. formosanus. Inhibition of either the Cfor-desatA2-a or Cfor-desatA2-b gene resulted in a significant decrease in the contents of fatty acids (C16:0, C18:0, C18:1 and C18:2) in worker castes. Moreover, we observed that inhibition of most of desaturase genes identified in this study had a negative impact on the survival rate and desiccation tolerance of workers. Interestingly, when normal soldiers were reared together with dsCfor-desatA2-b-treated workers, they exhibited higher mortality, suggesting that desaturase had an impact on trophallaxis among C. formosanus castes. Our findings shed light on the novel roles of desaturase family genes in the eusocial termite C. formosanus.

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第一去饱和酶亚家族基因对台湾白蚁脂肪酸合成、耐干燥性和种间营养转移的影响。

去饱和酶在不饱和脂肪酸（UFAs）的生物合成中起着重要作用。在本研究中，我们从台湾地下白蚁Coptotermes formosanus中鉴定了7个“第一去饱和酶”亚家族基因（Cfor-desatA1、Cfor-desatA2-a、Cfor-dsatA2-b、Cfor-DsatD和Cfor-desetE）。这些去饱和酶在台湾乳杆菌的角质层和脂肪体中高度表达。Cfor-desatA2-a或Cfor-desatA2-b基因的抑制导致工人种姓中脂肪酸含量（C16:0、C18:0、C18:1和C18:2）的显著降低。此外，我们观察到，本研究中鉴定的大多数去饱和酶基因的抑制对工人的存活率和干燥耐受性有负面影响。有趣的是，当正常士兵与dsCfor-desatA2处理的工人一起饲养时，他们表现出更高的死亡率，这表明去饱和酶对台湾乳杆菌种姓的营养不良有影响。我们的发现揭示了去饱和酶家族基因在真社会白蚁中的新作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).