Serum carotenoid levels are positively associated with DNA methylation of thioredoxin-interacting protein.

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Keisuke Maeda, Hiroya Yamada, Eiji Munetsuna, Ryosuke Fujii, Mirai Yamazaki, Yoshitaka Ando, Genki Mizuno, Yoshiki Tsuboi, Hiroaki Ishikawa, Koji Ohashi, Shuji Hashimoto, Nobuyuki Hamajima, Koji Suzuki
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引用次数: 0

Abstract

Background: Carotenoids have been reported to exert protective effects against age-related diseases via changes in DNA methylation. Although lower thioredoxin-interacting protein (TXNIP) DNA methylation is associated with age-related diseases, only a few studies have investigated the factors influencing TXNIP DNA methylation. Carotenoids may be a factor linking TXNIP to specific pathophysiological functions. The aim of this study was to examine whether serum carotenoid levels are associated with TXNIP DNA methylation levels. Methods: We conducted a cross-sectional study using 376 health examination participants (169 men). DNA methylation levels were determined using a pyrosequencing assay. Serum carotenoid levels were determined by high-performance liquid chromatography. Multivariable regression analyses were performed to examine the associations between TXNIP DNA methylation levels and serum carotenoid levels with adjustment for age, BMI, HbA1c, CRP, smoking habits, alcohol consumption, exercise habits, and percentage of neutrophils. Results: Multiple linear regression analyses showed that TXNIP DNA methylation levels were positively associated with serum levels of zeaxanthin/lutein (β [95%CI]: 1.935 [0.184, 3.685]), β-cryptoxanthin (1.447 [0.324, 2.570]), α-carotene (1.061 [0.044, 2.077]), β-carotene (1.272 [0.319, 2.226]), total carotenes (1.255 [0.040, 2.469]), total xanthophylls (2.133 [0.315, 3.951]), provitamin A (1.460 [0.402, 2.519]), and total carotenoids (1.972 [0.261, 3.683]) in men (all p<0.05). Of these, provitamin A showed the stronger association (standardized β=0.216). No significant association of TXNIP DNA methylation and serum carotenoid was observed in women. Conclusions: The findings of this study suggest that carotenoid intake may protect against age-related diseases by altering TXNIP DNA methylation status in men.

血清类胡萝卜素水平与硫氧还蛋白相互作用蛋白的DNA甲基化呈正相关。
背景:据报道,类胡萝卜素通过DNA甲基化的变化对与年龄相关的疾病发挥保护作用。尽管较低的硫氧还蛋白相互作用蛋白(TXNIP)DNA甲基化与年龄相关的疾病有关,但只有少数研究调查了影响TXNIP DNA甲基化的因素。类胡萝卜素可能是将TXNIP与特定病理生理功能联系起来的一个因素。本研究的目的是检测血清类胡萝卜素水平是否与TXNIP DNA甲基化水平有关。方法:我们对376名健康检查参与者(169名男性)进行了横断面研究。DNA甲基化水平使用焦磷酸测序测定法测定。通过高效液相色谱法测定血清类胡萝卜素水平。进行了多变量回归分析,以检查TXNIP DNA甲基化水平和血清类胡萝卜素水平之间的相关性,并对年龄、BMI、HbA1c、CRP、吸烟习惯、饮酒、运动习惯和中性粒细胞百分比进行了调整。结果:多元线性回归分析显示,TXNIP DNA甲基化水平与血清玉米黄质/叶黄素水平(β[95%CI]:1.935[0.1843.685])、β-隐黄质(1.447[0.3242.570])、α-胡萝卜素(1.061[0.0042.077])、,维生素A原(1.460[0.402,2.519])和类胡萝卜素总量(1.972[0.261,3.683])(均pβ=0.216)。女性TXNIP DNA甲基化与血清类胡萝卜素无显著相关性。结论:本研究结果表明,摄入类胡萝卜素可以通过改变男性TXNIP DNA甲基化状态来预防与年龄相关的疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.00
自引率
4.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: Since 1930 this journal has provided an important international forum for scientific advances in the study of nutrition and vitamins. Widely read by academicians as well as scientists working in major governmental and corporate laboratories throughout the world, this publication presents work dealing with basic as well as applied topics in the field of micronutrients, macronutrients, and non-nutrients such as secondary plant compounds. The editorial and advisory boards include many of the leading persons currently working in this area. The journal is of particular interest to: - Nutritionists - Vitaminologists - Biochemists - Physicians - Engineers of human and animal nutrition - Food scientists
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