First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-09-18 eCollection Date: 2023-01-01 DOI:10.1590/0074-02760230071

Felipe Dutra Rêgo, Eduardo Sérgio da Silva, Valeriana Valadares Lopes, Rafael Gonçalves Teixeira-Neto, Vinícius Silva Belo, Antônio Augusto Fonseca Júnior, Diego Andrade Pereira, Heber Paulino Pena, Márcia Dalastra Laurenti, Gabriela V Araújo, Vânia Lúcia Ribeiro da Matta, Islam Hussein Chouman, Thainá Bergantin Burrin, Carmen M Sandoval, Stella Maria Barrouin-Melo, Flaviane Alves de Pinho, Hélida Monteiro de Andrade, Ramon Vieira Nunes, Célia Maria Ferreira Gontijo, Vanete Thomaz Soccol, Donnamae Klocek, Danyil Grybchuk, Diego Henrique Macedo, Rubens Lima do Monte-Neto, Vyacheslav Yurchenko, Rodrigo Pedro Soares

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Abstract

Background: Leishmania RNA virus 1 (LRV1) is commonly found in South American Leishmania parasites belonging to the subgenus Viannia, whereas Leishmania RNA virus 2 (LRV2) was previously thought to be restricted to the Old-World pathogens of the subgenus Leishmania.

Objectives: In this study, we investigated the presence of LRV2 in strains of Leishmania (L.) infantum, the causative agent of visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions.

Methods: A total of seventy-one isolates were screened for LRV2 using semi-nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene.

Findings: We detected LRV2 in two L. infantum isolates (CUR268 and HP-EMO) from canine and human cases, respectively.

Main conclusions: To the best of our knowledge, this is the first detection of LRV2 in the New World.

Abstract Image

查看原文本刊更多论文

巴西东南部犬和人内脏利什曼病病例中婴儿利什曼原虫株中推定的利什曼原虫RNA病毒2（LRV2）的首次报告。

背景：利什曼原虫RNA病毒1（LRV1）通常在属于Viannia亚属的南美利什曼虫寄生虫中发现，而利什曼原虫RNA病毒2（LRV2）以前被认为仅限于旧世界利什曼虫亚属的病原体，内脏利什曼病（VL）的病原体，来源于不同的宿主、临床形式和地理区域。方法：采用半巢式逆转录聚合酶链反应（RT-PCR），以RNA依赖性RNA聚合酶（RdRp）基因为靶点，对71株分离株进行LRV2的筛选。研究结果：我们分别在犬和人的两个婴儿乳杆菌分离株（CUR268和HP-EMO）中检测到LRV2。主要结论：据我们所知，这是新世界首次检测到LRV2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.