The examination of some virulence factors in S. aureus isolates obtained from the healthy human population, sheep mastitis, and cheese.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2023-01-01 DOI:10.22099/IJVR.2023.43730.6410

L Rahbarnia, R Khosravi Rad, A R Dehnad, B Naghili

{"title":"The examination of some virulence factors in S. aureus isolates obtained from the healthy human population, sheep mastitis, and cheese.","authors":"L Rahbarnia, R Khosravi Rad, A R Dehnad, B Naghili","doi":"10.22099/IJVR.2023.43730.6410","DOIUrl":null,"url":null,"abstract":"Abstract.Background: Staphylococcus aureus is responsible for many infections in humans and animals from skin and soft tissue infections to life-threatening diseases. In this study to explore the origin of S. aureus infections in humans, the antibiotic resistance profile and the variety of virulence factors in S. aureus isolates were examined in three groups: a healthy human population, cheese, and the milk of sheep with mastitis.Aims: The examination of some virulence factors in S. aureus isolates obtained from the healthy human population, sheep mastitis, and cheese.Methods: A total of 400 nasal swab samples from healthy students, 30 cheese samples, and 122 sheep milk samples were collected for the detection of S. aureus isolates from January 1, 2018, to March 1, 2018. The frequency of hla, hlb, Acme/arcA, pvl, and tsst-1 virulence genes and mecA gene was determined in each group by PCR assay.Results: There was a direct relationship between the antibiotic susceptibility profile of the isolates from a healthy population and those from mastitis milk samples. Of 400 nasal samples, 15% (60/400) were positive for S. aureus, of which 60% (36/60) were positive for mecA. While 50% (15/30) of cheese samples were positive for S. aureus. of which 7 cases (46.66%, 7/15) were positive for mecA. The prevalence of S. aureus among students was dependent on gender (P=0.025). Also, 47.5% (58/122) of milk samples from sheep mastitis were positive for S. aureus, and 41.37% (24/58) were positive for the mecA gene. Based on PCR results, the highest rate of hla (68.33%, 41/60), hlb (53.33%, 32/60), and Acme/arcA (46.66%, 28/60) genes were related to a healthy population, and the highest frequency of pvl (41.38%, 24/58), and tsst-1 (27.59%, 16/58) was related to milk samples (P<0.05). A significant correlation was observed between the presence of the arginine catabolic mobile element (ACME)-arcA gene and resistance to methicillin (P<0.05).Conclusion: The high rate of virulence factors in the S. aureus isolates obtained from mastitis and dairy products is an alert point, because they could be source of the spreading of S. aureus to humans. There is an essential need for continuous monitoring to control staphylococcal food poisoning.","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"24 2","pages":"110-115"},"PeriodicalIF":0.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/81/52/ijvr-24-110.PMC10542877.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2023.43730.6410","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract.

Background: Staphylococcus aureus is responsible for many infections in humans and animals from skin and soft tissue infections to life-threatening diseases. In this study to explore the origin of S. aureus infections in humans, the antibiotic resistance profile and the variety of virulence factors in S. aureus isolates were examined in three groups: a healthy human population, cheese, and the milk of sheep with mastitis.

Aims: The examination of some virulence factors in S. aureus isolates obtained from the healthy human population, sheep mastitis, and cheese.

Methods: A total of 400 nasal swab samples from healthy students, 30 cheese samples, and 122 sheep milk samples were collected for the detection of S. aureus isolates from January 1, 2018, to March 1, 2018. The frequency of hla, hlb, Acme/arcA, pvl, and tsst-1 virulence genes and mecA gene was determined in each group by PCR assay.

Results: There was a direct relationship between the antibiotic susceptibility profile of the isolates from a healthy population and those from mastitis milk samples. Of 400 nasal samples, 15% (60/400) were positive for S. aureus, of which 60% (36/60) were positive for mecA. While 50% (15/30) of cheese samples were positive for S. aureus. of which 7 cases (46.66%, 7/15) were positive for mecA. The prevalence of S. aureus among students was dependent on gender (P=0.025). Also, 47.5% (58/122) of milk samples from sheep mastitis were positive for S. aureus, and 41.37% (24/58) were positive for the mecA gene. Based on PCR results, the highest rate of hla (68.33%, 41/60), hlb (53.33%, 32/60), and Acme/arcA (46.66%, 28/60) genes were related to a healthy population, and the highest frequency of pvl (41.38%, 24/58), and tsst-1 (27.59%, 16/58) was related to milk samples (P<0.05). A significant correlation was observed between the presence of the arginine catabolic mobile element (ACME)-arcA gene and resistance to methicillin (P<0.05).

Conclusion: The high rate of virulence factors in the S. aureus isolates obtained from mastitis and dairy products is an alert point, because they could be source of the spreading of S. aureus to humans. There is an essential need for continuous monitoring to control staphylococcal food poisoning.

Abstract Image

查看原文本刊更多论文

从健康人群、绵羊乳腺炎和奶酪中获得的金黄色葡萄球菌分离株中某些毒力因子的检测。

摘要：背景：金黄色葡萄球菌是许多人类和动物感染的原因，从皮肤和软组织感染到危及生命的疾病。在本研究中，为了探索人类金黄色葡萄球菌感染的起源，对三组金黄色葡萄菌分离株的抗生素耐药性和各种毒力因子进行了检测：健康人群、奶酪和患有乳腺炎的羊奶。目的：检测从健康人群、绵羊乳腺炎和奶酪中分离的金黄色葡萄球菌中的一些毒力因子。方法：从2018年1月1日至2018年3月1日，共采集健康学生的400份鼻拭子样本、30份奶酪样本和122份羊奶样本，用于检测金黄色葡萄球菌分离株。通过PCR测定各组中hla、hlb、Acme/arcA、pvl、tsst-1毒力基因和mecA基因的频率。结果：来自健康人群的分离株和来自乳腺炎牛奶样本的分离株的抗生素敏感性谱之间存在直接关系。在400份鼻腔样本中，15%（60/400）的金黄色葡萄球菌呈阳性，其中60%（36/60）的mecA呈阳性。而50%（15/30）的奶酪样品对金黄色葡萄球菌呈阳性。其中mecA阳性7例（46.66%）。金黄色葡萄球菌在学生中的患病率取决于性别（P=0.025）。此外，47.5%（58/122）的绵羊乳腺炎牛奶样本中金黄色葡萄菌呈阳性，41.37%（24/58）的mecA基因呈阳性。根据PCR结果，hla（68.33%，41/60）、hlb（53.33%，32/60）和Acme/arcA（46.66%，28/60）基因的最高比率与健康人群有关，pvl的最高频率（41.38%，24/58），tsst-1（27.59%，16/58）与牛奶样品有关（P.C）。结论：乳腺炎和乳制品分离的金黄色葡萄球菌中毒力因子的高比率是一个警报点，因为它们可能是金黄色葡萄菌向人类传播的来源。有必要持续监测以控制金黄色葡萄杆菌食物中毒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.