Expression of miR-145 and miR-18b in Peripheral Blood Samples of Head and Neck Cancer Patients.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Indian Journal of Clinical Biochemistry Pub Date : 2023-10-01 Epub Date: 2023-02-07 DOI:10.1007/s12291-023-01119-2

Arun Kumar Yadav, Nishant Singh, Sanjeev Kumar Yadav, M L B Bhatt, Amit Pandey, Dev Kumar Yadav, Sanjay Yadav

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引用次数: 0

Abstract

Head and neck squamous cell carcinomas (HNSCC) is one of the most prevalent type of cancer known in Indian population. Studies are needed to identify the early biomarkers for HNSCC. MicroRNAs (miRNAs) are non-coding RNA molecules, expression of which can be used as biomarker for early diagnosis of HNSCC. For miRNA profiling total RNA, which also contained small RNAs were isolated from ten HNSCC tissue samples and adjacent control. Purity and concentration of eluted RNA was assessed using the NanoDrop1000® spectrophotometer, Reverse Transcription reaction was carried out with megaplex RT primers of pool A and pool B and the expression of selected miRNAs (miR-143/145 and miR-18a/b) was measured using TaqMan primers specific for mature miRNAs. Our study showed dramatic downregulation in expression of two miRNAs, miR-18b and miR-145 in blood samples of HNSCC patients, which are inhibitor of tumorigenesis and can be targeted as biomarker of HNSCC pathogenesis therefore developing avenues for miRNA role in prognosis and therapeutics.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-023-01119-2.

查看原文本刊更多论文

头颈部癌症患者外周血样本中miR-145和miR-18b的表达。

头颈部鳞状细胞癌（HNSCC）是已知在印度人群中最常见的癌症类型之一。需要进行研究以确定HNSCC的早期生物标志物。微小RNA（miRNA）是非编码RNA分子，其表达可作为HNSCC早期诊断的生物标志物。对于miRNA图谱，从10个HNSCC组织样本和相邻对照中分离出总RNA，其中也含有小RNA。使用NanoDrop1000®分光光度计评估洗脱RNA的纯度和浓度，使用池A和池B的megaplex RT引物进行逆转录反应，并使用成熟miRNA特异性的TaqMan引物测量选定miRNA（miR-143/145和miR-18a/B）的表达。我们的研究显示，HNSCC患者血液样本中两种miRNA，miR-18b和miR-145的表达显著下调，这两种miRNAs是肿瘤发生的抑制剂，可以作为HNSCC发病机制的生物标志物，因此为miRNA在预后和治疗中的作用开辟了途径。补充信息：在线版本包含补充材料，请访问10.1007/s12291-023-0119-2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Clinical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

4.50

自引率

4.80%

发文量

期刊介绍： The primary mission of the journal is to promote improvement in the health and well-being of community through the development and practice of clinical biochemistry and dissemination of knowledge and recent advances in this discipline among professionals, diagnostics industry, government and non-government organizations. Indian Journal of Clinical Biochemistry (IJCB) publishes peer reviewed articles that contribute to the existing knowledge in all fields of Clinical biochemistry, either experimental or theoretical, particularly deal with the applications of biochemistry, molecular biology, genetics, biotechnology, and immunology to the diagnosis, treatment, monitoring and prevention of human diseases. The articles published also include those covering the analytical and molecular diagnostic techniques, instrumentation, data processing, quality assurance and accreditation aspects of the clinical investigations in which chemistry has played a major role, or laboratory animal studies with biochemical and clinical relevance.