Effect of crocin and quercetin supplementation in cryopreservation medium on post-thaw human sperm quality.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-01 Epub Date: 2023-09-30 DOI:10.1007/s10561-023-10110-3

Ensieh Salehi, Amir Shadboorestan, Afshin Mohammadi-Bardbori, Abedeh Mousavi, Elias Kargar-Abargouei, Parisa Sarkoohi, Mahmoud Omidi

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Abstract

Biochemical and physical changes during the cryopreservation process adversely affect sperm function required for fertilization. Recently, many studies have been conducted to find effective pre-freezing treatments to limit these damages. The present study aimed to investigate the effects of pre-freezing treatment with quercetin and crocin, individually or in combination, on sperm parameters after thawing procedure. For this, semen samples from 20 normozoospermic men were collected and then each sample was divided into five equal parts: 1. fresh group 2. frozen-thawed group without addition of antioxidants 3. frozen-thawed group containing 1 mM crocin, 4. frozen-thawed group containing 50 μM quercetin, and 5. frozen-thawed group containing a combination of 1 mM crocin and 50 μM quercetin. Pre-cryopreservation and post-thaw sperm motility, morphology, viability, DNA fragmentation, reactive oxygen species [1] (ROS) levels, and mitochondrial membrane potential [2] (MMP) were investigated. Cryopreservation significantly reduced sperm quality. Both crocin and quercetin individually improved sperm progressive motility, decreased ROS levels, reduced DNA fragmentation, and marginally increased MMP, though crocin seems to be more successful in protecting sperm quality. More interestingly, the combined addition of crocin and quercetin to the sperm-freezing medium did not show positive effects on sperm quality. Crocin and quercetin may play a role in mitigating the cryopreservation-induced injury to sperm.

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冷冻保存培养基中添加番红花苷和槲皮素对解冻后人类精子质量的影响。

冷冻保存过程中的生化和物理变化会对受精所需的精子功能产生不利影响。最近，已经进行了许多研究来寻找有效的冷冻前处理来限制这些损伤。本研究旨在研究槲皮素和番红花素单独或联合冷冻处理对解冻后精子参数的影响。为此，收集了20名精子正常男性的精液样本，然后将每个样本分为五等分：1。新鲜组2。不添加抗氧化剂的冻融组3。含有1mM番红花苷的冷冻-解冻组4。冻融组含槲皮素50μM。冷冻-解冻组，含有1mM番红花苷和50μM槲皮素的组合。研究了冷冻保存前和解冻后精子的活力、形态、活力、DNA断裂、活性氧[1]（ROS）水平和线粒体膜电位[2]（MMP）。冷冻保存显著降低精子质量。番红花苷和槲皮素分别改善了精子的进行性运动，降低了ROS水平，减少了DNA断裂，并略微增加了MMP，尽管番红花苷似乎在保护精子质量方面更成功。更有趣的是，在精子冷冻培养基中联合添加番红花苷和槲皮素并没有对精子质量产生积极影响。Crocin和槲皮素可能在减轻冷冻保存对精子的损伤中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.