Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways

IF 4.8 3区 化学 Q1 CHEMISTRY, MEDICINAL
Donatus Wewura Adongo, Charles Kwaku Benneh, Augustine Tandoh, Robert Peter Biney, Kennedy Kwami Edem Kukuia, Priscilla Kolibea Mante, Benjamin Kingsley Harley, David Oteng, Emmanuel Aduboffour Appiah, Ernest Cudjoe Anorbor, Eric Woode
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Abstract

Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases. The hydroethanolic extract (PME) from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models. However, its effects in chronic anxiety models and possible mechanism(s) of action were not studied. Therefore, the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety. The zebrafish light dark test (LDT) and novel tank test (NTT) were employed to assess the anxiolytic-like effects of PME (0.1, 0.3, 1.0 mg mL−1), fluoxetine (3 × 10−5 mg mL−1) and diazepam (1.5 × 10−7 mg mL−1). The chronic unpredictable stress (CUS) test was used to further evaluate the extract’s anxiolytic-like properties. The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil, granisetron, methysergide, or pizotifen, all at 1 × 10−3 mg mL−1. The extract significantly decreased anxiety behaviours in the NT and LD tests. These observed effects of the extract were however counteracted by flumazenil, granisetron, methysergide and pizotifen pre-treatment. In addition, PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish. Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.

Graphical abstract

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斑马鱼中微茶伪豆水乙醇叶提取物的类似焦虑作用:可能涉及GABA能和5-羟色胺能途径。
微小假丝酵母在民族医学中用于治疗中枢神经系统疾病。该植物叶子的水乙醇提取物(PME)在小鼠焦虑模型中显示出类似焦虑的特性。然而,它在慢性焦虑模型中的作用和可能的作用机制尚未研究。因此,本研究评估了PME在斑马鱼焦虑模型中的抗焦虑作用机制。斑马鱼明暗试验(LDT)和新型水槽试验(NTT)用于评估PME(0.1、0.3、1.0 mg mL-1)、氟西汀(3 × 10-5 mg mL-1)和地西泮(1.5 × 10-7mg mL-1)。使用慢性不可预测压力(CUS)测试来进一步评估提取物的抗焦虑特性。在用氟马西尼、格拉司琼、methysergide或吡唑替芬预处理后,对提取物抗焦虑作用的潜在机制进行了评估 × 10-3 mg mL-1。提取物显著降低了NT和LD测试中的焦虑行为。然而,观察到的提取物的这些作用被氟马西尼、格拉司琼、methysergide和吡唑替芬的预处理所抵消。此外,PME治疗显著逆转了CUS诱导的斑马鱼焦虑行为。结果表明,PME可能通过与5-羟色胺能和γ-氨基丁酸介导的途径相互作用而具有类似焦虑的作用。
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来源期刊
Natural Products and Bioprospecting
Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-
CiteScore
8.30
自引率
2.10%
发文量
39
审稿时长
13 weeks
期刊介绍: Natural Products and Bioprospecting serves as an international forum for essential research on natural products and focuses on, but is not limited to, the following aspects: Natural products: isolation and structure elucidation Natural products: synthesis Biological evaluation of biologically active natural products Bioorganic and medicinal chemistry Biosynthesis and microbiological transformation Fermentation and plant tissue cultures Bioprospecting of natural products from natural resources All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Natural Products and Bioprospecting publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of natural products. It is also an open access journal, which provides free access to its articles to anyone, anywhere.
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