Silencing of heat shock factor 1 (HSF1) inhibits proliferation, invasion, and epithelial-mesenchymal transition in oral squamous cell carcinoma

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2023-10-02 DOI:10.1111/jop.13491

Luiz Arthur Barbosa da Silva, Lucas Melo da Costa, Ana Camila Pereira Massetti, Laudenice de Lucena Pereira, Ericka Janine Dantas da Silveira, Tuula Anneli Salo, Ricardo Della Coletta, Márcia Cristina da Costa Miguel

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引用次数: 0

Abstract

Background

Oral squamous cell carcinoma is characterized by high rates of morbidity and mortality. Evidence obtained for different types of cancer shows that tumor initiation, progression, and therapeutic resistance are regulated by heat shock factor 1. This research aimed to analyze the effects of heat shock factor 1 on the biological behavior of oral squamous cell carcinoma.

Methods

Clinicopathological and immunoexpression study of heat shock factor 1 in 70 cases of oral tongue SCC and functional assays by gene silencing of this factor in an oral tongue SCC cell line.

Results

Heat shock factor 1 was overexpressed in oral tongue SCC specimens compared to normal oral mucosa (p < 0.0001) and in the SCC15 line compared to immortalized keratinocytes (p < 0.005). No significant associations were observed between overexpression of heat shock factor 1 and clinicopathological parameters or survival rates of the oral tongue SCC cases in the present sample. In vitro experiments showed that heat shock factor 1 silencing inhibited cell proliferation (p < 0.005) and cell cycle progression, with the accumulation of cells in the G0/G1 phase (p < 0.01). In addition, heat shock factor 1 silencing reduced cell invasion capacity (p < 0.05) and epithelial-mesenchymal transition, characterized by a decrease in vimentin expression (p < 0.05) and an increase in E-cadherin expression (p < 0.001).

Conclusion

Heat shock factor 1 may exert several functions that help maintain cell stability under the stressful conditions of the tumor microenvironment. Thus, strategies targeting the regulation of this protein may in the future be a useful therapeutic tool to control the progression of oral squamous cell carcinoma.

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热休克因子1（HSF1）的沉默抑制口腔鳞状细胞癌的增殖、侵袭和上皮-间质转化。

背景：口腔鳞状细胞癌的特点是发病率和死亡率高。获得的不同类型癌症的证据表明，肿瘤的发生、发展和治疗耐药性受热休克因子1的调节。本研究旨在分析热休克因子1对口腔鳞状细胞癌生物学行为的影响。方法：对70例口腔鳞状细胞癌患者热休克因子1的临床病理和免疫表达进行研究，并通过基因沉默对该因子进行功能测定。结果：与正常口腔黏膜相比，热休克因子1在口腔舌鳞状细胞癌中过表达（p 结论：热休克因子1可能在肿瘤微环境的应激条件下发挥多种功能，帮助维持细胞的稳定性。因此，靶向该蛋白调控的策略可能在未来成为控制口腔鳞状细胞癌进展的有用治疗工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.