Co-inoculation timing affects the interspecific interactions between phoma stem canker pathogens Leptosphaeria maculans and Leptosphaeria biglobosa

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2023-09-27 DOI:10.1002/ps.7799

Evren Bingol, Aiming Qi, Chinthani Karandeni-Dewage, Faye Ritchie, Bruce D. L. Fitt, Yong-Ju Huang

{"title":"Co-inoculation timing affects the interspecific interactions between phoma stem canker pathogens Leptosphaeria maculans and Leptosphaeria biglobosa","authors":"Evren Bingol, Aiming Qi, Chinthani Karandeni-Dewage, Faye Ritchie, Bruce D. L. Fitt, Yong-Ju Huang","doi":"10.1002/ps.7799","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>Phoma stem canker is an economically important disease of oilseed rape, caused by two co-existing fungal pathogen species, <i>Leptosphaeria maculans</i> (<i>Plenodomus lingam</i>) and <i>Leptosphaeria biglobosa</i> (<i>Plenodomus biglobosus</i>). <i>Leptosphaeria maculans</i> produces a phytotoxin called sirodesmin PL. Our previous work showed that <i>L. biglobosa</i> has an antagonistic effect on the production of sirodesmin PL if it is simultaneously co-inoculated with <i>L. maculans</i>. However, the effects of sequential co-inoculation on interspecific interactions between the two pathogens are not understood.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>The interactions between <i>L. maculans</i> and <i>L. biglobosa</i> were investigated in liquid culture by inoculation with <i>L. maculans</i> first, followed by <i>L. biglobosa</i> sequentially at 1, 3, 5 or 7 days later and vice versa; the controls were inoculated with <i>L. maculans</i> only, <i>L. biglobosa</i> only, or <i>L. maculans</i> and <i>L. biglobosa</i> simultaneously. The results showed that <i>L. biglobosa</i> inhibited the growth of <i>L. maculans</i>, the production of both sirodesmin PL and its precursors if <i>L. biglobosa</i> was inoculated before, or simultaneously with, <i>L. maculans</i>. However, the antagonistic effects of <i>L. biglobosa</i> were lost if it was co-inoculated 5 or 7 days after <i>L. maculans</i>.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSION</h3>\n \n <p>For the first time, the results of this study provided evidence that the timing when <i>L. maculans</i> and <i>L. biglobosa</i> meet significantly influences the outcome of the interspecific competition between them. <i>Leptosphaeria biglobosa</i> can inhibit the production of sirodesmin PL and the growth of <i>L. maculans</i> if it is inoculated before <i>L. maculans</i> or less than 3 days after <i>L. maculans</i> in liquid culture. There is a need to further investigate the timing of co-inoculation on interactions between <i>L. maculans</i> and <i>L. biglobosa</i> in their host plants for improving the control of phoma stem canker. © 2023 The Authors. <i>Pest Management Science</i> published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>\n </section>\n </div>","PeriodicalId":218,"journal":{"name":"Pest Management Science","volume":"80 5","pages":"2443-2452"},"PeriodicalIF":3.8000,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ps.7799","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pest Management Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ps.7799","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

BACKGROUND

Phoma stem canker is an economically important disease of oilseed rape, caused by two co-existing fungal pathogen species, Leptosphaeria maculans (Plenodomus lingam) and Leptosphaeria biglobosa (Plenodomus biglobosus). Leptosphaeria maculans produces a phytotoxin called sirodesmin PL. Our previous work showed that L. biglobosa has an antagonistic effect on the production of sirodesmin PL if it is simultaneously co-inoculated with L. maculans. However, the effects of sequential co-inoculation on interspecific interactions between the two pathogens are not understood.

RESULTS

The interactions between L. maculans and L. biglobosa were investigated in liquid culture by inoculation with L. maculans first, followed by L. biglobosa sequentially at 1, 3, 5 or 7 days later and vice versa; the controls were inoculated with L. maculans only, L. biglobosa only, or L. maculans and L. biglobosa simultaneously. The results showed that L. biglobosa inhibited the growth of L. maculans, the production of both sirodesmin PL and its precursors if L. biglobosa was inoculated before, or simultaneously with, L. maculans. However, the antagonistic effects of L. biglobosa were lost if it was co-inoculated 5 or 7 days after L. maculans.

CONCLUSION

For the first time, the results of this study provided evidence that the timing when L. maculans and L. biglobosa meet significantly influences the outcome of the interspecific competition between them. Leptosphaeria biglobosa can inhibit the production of sirodesmin PL and the growth of L. maculans if it is inoculated before L. maculans or less than 3 days after L. maculans in liquid culture. There is a need to further investigate the timing of co-inoculation on interactions between L. maculans and L. biglobosa in their host plants for improving the control of phoma stem canker. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Abstract Image

查看原文本刊更多论文

共同接种时间影响光茎溃疡病原体细粉藻和大球藻之间的种间相互作用。

背景：Phoma茎溃疡病是一种经济上重要的油菜病害，由两种共同存在的真菌致病菌Leptosphaeria maculans（Pleodomus lingam）和L.biglobosa（P.biglobosus）引起。黄斑乳杆菌产生一种名为西罗地霉素PL的植物毒素。我们之前的工作表明，如果同时与黄斑乳杆菌共同接种，大球乳杆菌对西罗地菌素PL的产生具有拮抗作用。然而，顺序共接种对两种病原体之间种间相互作用的影响尚不清楚。结果：在液体培养基中，首先接种斑点乳杆菌，然后在第1、3、5或7天依次接种大叶乳杆菌，研究了斑点乳杆菌和大叶乳杆菌之间的相互作用天之后，反之亦然；对照组仅接种斑点乳杆菌，仅接种大球乳杆菌，或同时接种斑乳杆菌和大球乳球菌。结果表明，如果在斑点乳杆菌之前或同时接种斑点乳杆菌，则斑点乳杆菌可抑制斑点乳杆菌的生长，抑制西罗莫司明PL及其前体的产生。然而，如果共同接种5或7，则大球乳杆菌的拮抗作用消失斑节乳杆菌后数天。结论：本研究的结果首次提供了证据，表明斑点乳杆菌和大叶乳杆菌相遇的时间对它们之间种间竞争的结果有显著影响。如果在斑点乳杆菌之前接种或少于3次，则大球乳杆菌可以抑制西罗司明PL的产生和斑点乳杆菌的生长在液体培养中的斑乳杆菌培养后第天。有必要进一步研究共同接种的时间对斑点乳杆菌和大叶乳杆菌在其寄主植物中的相互作用，以改善对光茎溃疡的控制。这篇文章受版权保护。保留所有权利。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.