Osteoblastogenesis and osteolysis in the Zucker Diabetic Sprague Dawley rat humerus head.

IF 1.4 Q3 ANATOMY & MORPHOLOGY
Anatomy & Cell Biology Pub Date : 2023-12-31 Epub Date: 2023-10-04 DOI:10.5115/acb.23.166
Gcwalisile Frances Dlamini, Robert Ndou
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引用次数: 0

Abstract

The endocrinology of type 2 diabetes (T2D) and its predisposing factors have been studied extensively while its skeletal effects have received negligible research despite this being a global disease. The cellular and molecular association between proximal humeral fractures and T2D has not been fully elucidated. We aimed to study bone cell quantities and immunolabel osteogenic and antiosteogenic cytokines. The study used 12-week-old rats (23 males) consisting of 8 Sprague Dawley (SD) and 15 Zucker Diabetic Sprague Dawley (ZDSD). Weekly mass measurements were taken while fasting blood glucose levels were recorded every 2 weeks with oral glucose tolerance tests conducted once every 4 weeks. Upon termination at the age of 28 weeks, humeri were fixed in 10% buffered formalin, prior to decalcification in ethylenediaminetetraacetic acid. The bone samples were then processed in ascending grades of alcohol using an automatic processor before embedding in paraffin wax. Sections were cut at 5 μm thickness in a series for Haematoxylin and Eosin stain, and immunohistochemistry was performed with the anti-tartrate-resistant acid phosphatase (TRAP), anti-alkaline phosphatase (ALP), anti-bone morphogenetic protein 3 (BMP3), anti-transforming growth factor beta 1 (TGFβ1), anti-aged glycation end product (AGE) antibodies in the sequence. ZDSD rats had more adipocytes, BMP3 and AGEs expression with higher numbers of TRAP positive osteocytes and fewer ALP cells although no differences were found in TGFβ1 immunopositivity. We also found that T2D increases the number of AGEs immuno-positive cells, as well as its extracellular expression, thus providing a conducive environment for the interaction of the osteogenic cytokine and its antagonist to suppress osteoblastogenesis. ZDSD groups had higher adipocyte numbers therefore increased marrow adiposity in T2D.

Zucker糖尿病Sprague-Dawley大鼠肱骨头的成骨细胞生成和骨溶解。
2型糖尿病(T2D)的内分泌学及其诱发因素已被广泛研究,而其骨骼影响的研究却微不足道,尽管这是一种全球性疾病。肱骨近端骨折与T2D之间的细胞和分子相关性尚未完全阐明。我们的目的是研究骨细胞的数量和免疫标记的成骨和抗骨细胞因子。该研究使用了12周大鼠(23只雄性),包括8只Sprague-Dawley(SD)和15只Zucker糖尿病Sprague-Dawley(ZDSD)。每周进行质量测量,同时每2周记录空腹血糖水平,每4周进行一次口服葡萄糖耐量测试。在28周龄终止时,在乙二胺四乙酸中脱钙之前,将humeri固定在10%缓冲福尔马林中。然后,在嵌入石蜡之前,使用自动处理器在递增等级的酒精中对骨骼样本进行处理。切片厚度为5µm,用于苏木精和曙红染色,并用序列中的抗酒石酸抗性酸性磷酸酶(TRAP)、抗碱性磷酸酶(ALP)、骨形态发生蛋白3(BMP3)、抗转化生长因子β1(TGFβ1)和抗衰老糖基化终产物(AGE)抗体进行免疫组织化学。ZDSD大鼠有更多的脂肪细胞、BMP3和AGEs表达,TRAP阳性骨细胞数量更高,ALP细胞更少,尽管TGFβ1的免疫阳性率没有差异。我们还发现,T2D增加了AGEs免疫阳性细胞的数量及其细胞外表达,从而为成骨细胞因子及其拮抗剂的相互作用提供了有利的环境,以抑制成骨细胞的生成。ZDSD组具有较高的脂肪细胞数量,因此增加了T2D患者的骨髓肥胖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Anatomy & Cell Biology
Anatomy & Cell Biology ANATOMY & MORPHOLOGY-
CiteScore
1.80
自引率
9.10%
发文量
75
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