Dynamic patterns of histone lactylation during early tooth development in mice

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2023-10-03 DOI:10.1007/s10735-023-10154-5

Ming Liu, Qian Yang, Huanyan Zuo, Xinye Zhang, Yuji Mishina, Zhi Chen, Jingwen Yang

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Abstract

Histone lactylation on its lysine (K) residues has been reported to have indispensable roles in lung fibrosis, embryogenesis, neural development, inflammation, and tumors. However, little is known about the lactylation activity towards histone lysine residue during tooth development. We investigated the dynamic patterns of lactate-derived histone lysine lactylation (Kla) using a pan-Kla antibody during murine tooth development, including lower first molar and lower incisor. The results showed that pan-Kla exhibited temporo-spatial patterns in both dental epithelium and mesenchyme cells during development. Notably, pan-Kla was identified in primary enamel knot (PEK), stratum intermedium (SI), stellate reticulum (SR), dental follicle cells, odontoblasts, ameloblasts, proliferating cells in dental mesenchyme, as well as osteoblasts around the tooth germ. More importantly, pan-Kla was also identified to be co-localized with neurofilament during tooth development, suggesting histone lysine lactylation may be involved in neural invasion during tooth development. These findings suggest that histone lysine lactylation may play important roles in regulating tooth development.

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小鼠早期牙齿发育过程中组蛋白乳酸化的动态模式。

据报道，赖氨酸（K）残基上的组蛋白乳酸化在肺纤维化、胚胎发生、神经发育、炎症和肿瘤中发挥着不可或缺的作用。然而，在牙齿发育过程中，对组蛋白赖氨酸残基的乳酸化活性知之甚少。我们使用泛Kla抗体研究了小鼠牙齿发育过程中，包括下第一磨牙和下切牙，乳酸盐衍生的组蛋白赖氨酸乳酸化（Kla）的动态模式。结果表明，泛Kla在发育过程中在牙上皮和间充质细胞中均表现出颞空间模式。值得注意的是，泛Kla在原发性釉质结（PEK）、中间层（SI）、星状网（SR）、牙毛囊细胞、成牙本质细胞、成釉细胞、牙间质增殖细胞以及牙胚周围的成骨细胞中被鉴定。更重要的是，在牙齿发育过程中，泛Kla也被鉴定为与神经丝共定位，这表明组蛋白赖氨酸乳酸化可能参与了牙齿发育过程的神经侵袭。这些发现表明，组蛋白赖氨酸乳酸化可能在调节牙齿发育中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.