Epigenetic regulation of female germline development through ERECTA signaling pathway

IF 9.4 1区生物学 Q1 Agricultural and Biological Sciences

New Phytologist Pub Date : 2023-08-22 DOI:10.1111/nph.19217

Youmei Huang, Liping Liu, Mengnan Chai, Han Su, Suzhuo Ma, Kaichuang Liu, Yaru Tian, Zhuangyuan Cao, Xinpeng Xi, Wenhui Zhu, Jingang Qi, Ravishankar Palanivelu, Yuan Qin, Hanyang Cai

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引用次数: 0

Abstract

Germline development is a key step in sexual reproduction. Sexual plant reproduction begins with the formation of haploid spores by meiosis of megaspore mother cells (MMCs). Although many evidences, directly or indirectly, show that epigenetics plays an important role in MMC specification, how it controls the commitment of the MMC to downstream stages of germline development is still unclear.
Electrophoretic mobility shift assay (EMSA), western blot, immunofluorescence, and chromatin immunoprecipitation coupled with quantitative PCR analyses were performed. Genetic interactions between BZR1 transcription factor family and the SWR1-SDG2-ER pathway in the control of female germline development were further studied.
The present findings showed in Arabidopsis that two epigenetic factors, the chromatin remodeling complex SWI2/SNF2-RELATED 1 (SWR1) and a writer for H3K4me3 histone modification SET DOMAIN GROUP 2 (SDG2), genetically interact with the ERECTA (ER) receptor kinase signaling pathway and regulate female germline development by restricting the MMC cell fate to a single cell in the ovule primordium and ensure that only that single cell undergoes meiosis and subsequent megaspore degeneration. We also showed that SWR1-SDG2-ER signaling module regulates female germline development by promoting the protein accumulation of BZR1 transcription factor family on the promoters of primary miRNA processing factors, HYPONASTIC LEAVES 1 (HYL1), DICER-LIKE 1 (DCL1), and SERRATE (SE) to activate their expression.
Our study elucidated a Gene Regulation Network that provides new insights for understanding how epigenetic factors and receptor kinase signaling pathways function in concert to control female germline development in Arabidopsis.

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ERECTA信号通路对雌性生殖系发育的表观遗传学调控

生殖系发育是有性生殖的关键步骤。植物有性繁殖始于大孢子母细胞减数分裂形成单倍体孢子。尽管许多证据直接或间接表明表观遗传学在MMC规范中发挥着重要作用，但它如何控制MMC对种系发育下游阶段的承诺仍不清楚。进行电泳迁移率转移分析（EMSA）、蛋白质印迹、免疫荧光和染色质免疫沉淀结合定量PCR分析。进一步研究了BZR1转录因子家族与SWR1-SDG2-ER通路在控制雌性生殖系发育中的遗传相互作用。目前的研究结果表明，在拟南芥中，两种表观遗传因子——染色质重塑复合物SWI2/SNF2-RATED 1（SWR1）和H3K4me3组蛋白修饰SET DOMAIN GROUP 2（SDG2）的作者，与ERECTA（ER）受体激酶信号通路遗传相互作用，并通过将MMC细胞命运限制在胚珠原基中的单个细胞来调节雌性生殖系发育，并确保只有该单个细胞经历减数分裂和随后的大孢子变性。我们还发现，SWR1-SDG2-ER信号模块通过促进BZR1转录因子家族在初级miRNA处理因子、缺氧叶1（HYL1）、二细胞样1（DCL1）和SERRATE（SE）启动子上的蛋白质积累来调节雌性生殖系发育，以激活其表达。我们的研究阐明了一个基因调控网络，该网络为理解表观遗传因子和受体激酶信号通路如何协同控制拟南芥雌性生殖系发育提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist PLANT SCIENCES-

CiteScore

17.60

自引率

5.30%

发文量

728

审稿时长

1 months

期刊介绍： New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.