Leptin receptor expression in blood mononuclear cells of lactating women is associated with infant body weight: Potential role as a molecular biomarker

IF 2.7 3区医学 Q1 PEDIATRICS

Pediatric Obesity Pub Date : 2023-08-31 DOI:10.1111/ijpo.13072

Agustina Malpeli, María Victoria Fasano, Marisa Sala, Pablo Obregón, Carla Casado, Ignacio Mendez, Lucrecia Fotia, Daniel Castrogiovanni, Ana Varea, Liliana Disalvo, Andrea Tournier, Lucía Mazziota, Daniela Rocha, Ana Luz Kruger, Laura Orellano, María F. Andreoli

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引用次数: 0

Abstract

Background

Molecular biomarkers of maternal leptin resistance associated with infant weight are needed.

Objectives

To evaluate gene expression of leptin receptor (LEPR), suppressor of cytokine signalling 3 (SOCS3) and insulin receptor in peripheral blood mononuclear cells (PBMCs) of lactating women and their relationship with infant body weight and adiposity.

Methods

At day 10 postpartum, maternal gene expression in PBMCs as well as leptin and insulin concentrations in plasma and milk were assessed (n = 68). Infant weight and BMI z-scores, skinfolds and arm circumference were obtained at 10 days and/or at 3 months old.

Results

In mothers with pre-pregnancy overweight or obesity (OW/OB), LEPR expression was reduced (p = 0.013) whereas plasma and milk leptin and milk insulin concentrations were elevated. LEPR expression was positively related with infant weight z-score (Beta (95% CI): 0.40 (0.17, 0.63), p = 0.001) but not with leptin concentrations. SOCS3 expression was positively related with infant weight z-score (Beta (95% CI): 0.28 (0.04, 0.51), p = 0.024) and arm circumference (Beta (95% CI): 0.57 (0.32, 0.82), p < 0.001). Relationships remained significant after adjusting for maternal and infant confounders.

Conclusions

LEPR and SOCS3 gene expression in PBMCs are novel maternal molecular biomarkers that reflect leptin resistance and are associated with infant body weight and adiposity.

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哺乳期妇女血单个核细胞中瘦素受体的表达与婴儿体重有关：作为分子生物标志物的潜在作用

背景需要与婴儿体重相关的母体瘦素抵抗的分子生物标志物。目的探讨哺乳期妇女外周血单个核细胞（PBMC）瘦素受体（LEPR）、细胞因子信号抑制因子3（SOCS3）和胰岛素受体的基因表达及其与婴儿体重和肥胖的关系。方法在产后10天，测定母亲外周血单个核细胞的基因表达以及血浆和乳汁中瘦素和胰岛素的浓度（n = 68）。婴儿体重和BMI z评分、皮褶和臂围在10岁时获得天和/或在3个月大时。结果在孕前超重或肥胖（OW/OB）的母亲中，LEPR的表达降低（p = 0.013），而血浆和乳汁瘦素和乳汁胰岛素浓度升高。LEPR表达与婴儿体重z评分呈正相关（β（95%置信区间）：0.40（0.17，0.63），p = 0.001），但与瘦素浓度无关。SOCS3的表达与婴儿体重z评分呈正相关（β（95%置信区间）：0.28（0.04，0.51），p = 0.024）和臂围（β（95%CI）:0.57（0.32，0.82），p <； 0.001）。在调整了母婴混杂因素后，关系仍然显著。结论LEPR和SOCS3基因在PBMC中的表达是反映瘦素抵抗的新的母体分子生物标志物，与婴儿体重和肥胖有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pediatric Obesity PEDIATRICS-

CiteScore

7.30

自引率

5.30%

发文量

117

审稿时长

6-12 weeks

期刊介绍： Pediatric Obesity is a peer-reviewed, monthly journal devoted to research into obesity during childhood and adolescence. The topic is currently at the centre of intense interest in the scientific community, and is of increasing concern to health policy-makers and the public at large. Pediatric Obesity has established itself as the leading journal for high quality papers in this field, including, but not limited to, the following: Genetic, molecular, biochemical and physiological aspects of obesity – basic, applied and clinical studies relating to mechanisms of the development of obesity throughout the life course and the consequent effects of obesity on health outcomes Metabolic consequences of child and adolescent obesity Epidemiological and population-based studies of child and adolescent overweight and obesity Measurement and diagnostic issues in assessing child and adolescent adiposity, physical activity and nutrition Clinical management of children and adolescents with obesity including studies of treatment and prevention Co-morbidities linked to child and adolescent obesity – mechanisms, assessment, and treatment Life-cycle factors eg familial, intrauterine and developmental aspects of child and adolescent obesity Nutrition security and the "double burden" of obesity and malnutrition Health promotion strategies around the issues of obesity, nutrition and physical activity in children and adolescents Community and public health measures to prevent overweight and obesity in children and adolescents.