Rice chromatin protein OsHMGB1 is involved in phosphate homeostasis and plant growth by affecting chromatin accessibility

IF 9.4 1区生物学 Q1 Agricultural and Biological Sciences

New Phytologist Pub Date : 2023-08-08 DOI:10.1111/nph.19189

Yan Wang, Fei Wang, Hong Lu, Rongbin Lin, Jiaming Liu, Yu Liu, Jiming Xu, Yunrong Wu, Zhiye Wang, Ming Zhou, Xiaorong Mo, Zhongchang Wu, Huixia Shou, Shaojian Zheng, Chuanzao Mao

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引用次数: 0

Abstract

Although phosphorus is one of the most important essential elements for plant growth and development, the epigenetic regulation of inorganic phosphate (Pi) signaling is poorly understood.
In this study, we investigated the biological function and mode of action of the high-mobility-group box 1 protein OsHMGB1 in rice (Oryza sativa), using molecular and genetic approaches.
We determined that OsHMGB1 expression is induced by Pi starvation and encodes a nucleus-localized protein. Phenotypic analysis of Oshmgb1 mutant and OsHMGB1 overexpression transgenic plants showed that OsHMGB1 positively regulates Pi homeostasis and plant growth. Transcriptome deep sequencing and chromatin immunoprecipitation followed by sequencing indicated that OsHMGB1 regulates the expression of a series of phosphate starvation–responsive (PSR) genes by binding to their promoters. Furthermore, an assay for transposase-accessible chromatin followed by sequencing revealed that OsHMGB1 is involved in maintaining chromatin accessibility. Indeed, OsHMGB1 occupancy positively correlated with genome-wide chromatin accessibility and gene expression levels.
Our results demonstrate that OsHMGB1 is a transcriptional facilitator that regulates the expression of a set of PSR genes to maintain Pi homeostasis in rice by increasing the chromatin accessibility, revealing a key epigenetic mechanism that fine-tune plant acclimation responses to Pi-limited environments.

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水稻染色质蛋白OsHMGB1通过影响染色质可及性参与磷酸盐稳态和植物生长

尽管磷是植物生长发育最重要的必需元素之一，但人们对无机磷（Pi）信号传导的表观遗传学调控知之甚少。在本研究中，我们利用分子和遗传学方法研究了高迁移率box 1蛋白OsHMGB1在水稻中的生物学功能和作用模式。我们确定OsHMGB1的表达是由Pi饥饿诱导的，并编码细胞核定位蛋白。Oshmgb1突变体和Oshmgb1过表达转基因植物的表型分析表明，OsHMGB11正调节Pi稳态和植物生长。转录组深度测序和染色质免疫沉淀后测序表明，OsHMGB1通过与一系列磷酸饥饿反应（PSR）基因的启动子结合来调节其表达。此外，转座酶可及染色质的测定和测序显示OsHMGB1参与维持染色质的可及性。事实上，OsHMGB1的占有率与全基因组染色质的可及性和基因表达水平呈正相关。我们的研究结果表明，OsHMGB1是一种转录促进因子，它通过增加染色质的可及性来调节一组PSR基因的表达，以维持水稻的Pi稳态，揭示了一种关键的表观遗传学机制，可以微调植物对Pi限制环境的适应反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist PLANT SCIENCES-

CiteScore

17.60

自引率

5.30%

发文量

728

审稿时长

1 months

期刊介绍： New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.