Biodegradation of di-2-ethylhexyl phthalate by Bacillus firmus MP04 strain: parametric optimization using full factorial design

IF 3.1 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2023-06-24 DOI:10.1007/s10532-023-10043-4

Madhavi Rashmi, Tanuja Singh, Nitesh Singh Rajput, Shweta Kulshreshtha

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引用次数: 0

Abstract

Di-2-ethylhexyl phthalate (DEHP) is used as a plasticizer in making plastics and released from landfills. This study attempted to degrade DEHP using microbial isolates. Isolates of Bacillus spp. were tested for their efficacy in degrading DEHP. Degradation was assessed using liquid chromatography-mass spectrometry (LC–MS). The most efficient DEHP degradation was achieved by Bacillus firmus MP04, which has been identified as Bacillus firmus MP04. This strain was found to use DEHP as the sole source of carbon without carbon source supplementation. Full factorial design was used to optimize the conditions for DEHP degradation which revealed the suitability of pH 7, 5% salt concentration, 20 to 37 °C temperature, and yeast extract as a nitrogen source. LC–MS elucidated the possible degradation mechanism via benzoic acid formation. However, prolonged incubation formed a typical compound denatonium benzoate due to reactions with other compounds. As maximum degradation was achieved in 4 days, prolonged incubation is not suggested. It can be concluded that new strain Bacillus firmus MP04 is the most efficient strain among all the tested strains for DEHP degradation.

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固体芽孢杆菌MP04菌株降解邻苯二甲酸二-2-乙基己酯的全因子设计参数优化

邻苯二甲酸二-2-乙基己酯（DEHP）被用作制造塑料的增塑剂，并从垃圾填埋场释放。本研究试图利用微生物分离物降解DEHP。测试了芽孢杆菌的分离物对DEHP的降解效果。使用液相色谱-质谱法（LC–MS）评估降解情况。最有效的DEHP降解是由坚固芽孢杆菌MP04实现的，其已被鉴定为坚固芽孢杆菌MP04。发现该菌株在不补充碳源的情况下使用DEHP作为唯一的碳源。采用全因子设计优化了DEHP的降解条件，结果表明pH为7、盐浓度为5%、温度为20至37°C以及酵母提取物作为氮源是合适的。LC–MS阐明了通过苯甲酸形成的可能降解机制。然而，由于与其他化合物的反应，长时间的孵育形成了典型的苯甲酸癸铵化合物。由于在4天内达到最大降解，因此不建议延长培养时间。可以得出结论，在所有测试菌株中，新菌株坚定芽孢杆菌MP04是降解DEHP最有效的菌株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.