Acidovorax PSJ13, a novel, efficient polyacrylamide-degrading bacterium by cleaving the main carbon chain skeleton without the production of acrylamide

IF 3.1 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2023-07-03 DOI:10.1007/s10532-023-10036-3

Zhengjiang Wang, Kaili Li, Xuwei Gui, Zhenlun Li

{"title":"Acidovorax PSJ13, a novel, efficient polyacrylamide-degrading bacterium by cleaving the main carbon chain skeleton without the production of acrylamide","authors":"Zhengjiang Wang, Kaili Li, Xuwei Gui, Zhenlun Li","doi":"10.1007/s10532-023-10036-3","DOIUrl":null,"url":null,"abstract":"<div><p>Given the environmental challenge caused by the wide use of polyacrylamide (PAM), an environmental-friendly treatment method is required. This study demonstrates the role of <i>Acidovorax</i> sp. strain PSJ13 isolated from dewatered sludge in efficiently degrading PAM. To be specific, the strain PSJ13 can degrade 51.67% of PAM in 96 h (2.39 mg/(L h)) at 35 °C, pH 7.5 and 5% inoculation amount. Besides, scanning electron microscope, X-ray photoelectron spectroscopy, liquid chromatography–mass spectrometry and high-performance liquid chromatography were employed to analyze samples, and the nitrogen present in the degradation products was investigated. The results showed that the degradation of PAM by PSJ13 started from the side chain and then mainly the –C–C– main chain, which produced no acrylamide monomers. As the first study to report the role of <i>Acidovorax</i> in efficiently degrading PAM, this work may provide a solution for industries that require PAM management.</p><h3>Graphical abstract</h3>\n <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\n </div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"34 6","pages":"581 - 595"},"PeriodicalIF":3.1000,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biodegradation","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10532-023-10036-3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Given the environmental challenge caused by the wide use of polyacrylamide (PAM), an environmental-friendly treatment method is required. This study demonstrates the role of Acidovorax sp. strain PSJ13 isolated from dewatered sludge in efficiently degrading PAM. To be specific, the strain PSJ13 can degrade 51.67% of PAM in 96 h (2.39 mg/(L h)) at 35 °C, pH 7.5 and 5% inoculation amount. Besides, scanning electron microscope, X-ray photoelectron spectroscopy, liquid chromatography–mass spectrometry and high-performance liquid chromatography were employed to analyze samples, and the nitrogen present in the degradation products was investigated. The results showed that the degradation of PAM by PSJ13 started from the side chain and then mainly the –C–C– main chain, which produced no acrylamide monomers. As the first study to report the role of Acidovorax in efficiently degrading PAM, this work may provide a solution for industries that require PAM management.

Graphical abstract

Abstract Image

查看原文本刊更多论文

新型高效聚丙烯酰胺降解菌PSJ13通过裂解主碳链骨架而不产生丙烯酰胺

鉴于聚丙烯酰胺（PAM）的广泛使用对环境造成的挑战，需要一种环保的处理方法。本研究证明了从脱水污泥中分离的Acidovorax sp.菌株PSJ13在高效降解PAM中的作用。具体而言，菌株PSJ13在35°C、pH 7.5和5%接种量下，可在96小时内降解51.67%的PAM（2.39 mg/（L h））。此外，还采用扫描电子显微镜、X射线光电子能谱、液相色谱-质谱和高效液相色谱对样品进行了分析，并对降解产物中的氮进行了研究。结果表明，PSJ13对PAM的降解从侧链开始，然后主要从–C–C–主链开始，不产生丙烯酰胺单体。作为第一项报道阿昔洛韦在高效降解PAM中作用的研究，这项工作可能为需要PAM管理的行业提供解决方案。图形摘要

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.