{"title":"Validation of two enzyme immunoassays for aminoglycoside residues according to European Decision 657/2002.","authors":"Francesca Diana, Maurizio Paleologo, Lidija Persic","doi":"10.1080/02652030701458097","DOIUrl":null,"url":null,"abstract":"<p><p>Aminoglycoside antibiotics are commonly used to treat bacterial infections in human and veterinary practice. Owing to their toxicity, the European Community has established maximum residue limits (MRL) in foodstuffs of animal origin (EEC No 2377/90). In the present study, the performance of two new enzyme immunoassays (EIA), I'screen Gentamicin and I'screen Neomycin, for the quantitative detection of the aminoglycosides, gentamicin and neomycin, in milk and tissue are described. Validation of these EIAs has been performed in accordance to the criteria of European Decision 657/2002. Assays sensitivity at the MRLs was 95% for milk samples and 100% for tissue samples, while specificity was 100% at 33 and 25% of the MRLs for milk and tissues, respectively. The performance of these EIAs indicates that they can be used as easy screening methods for the analysis of aminoglycosides in milk and tissue samples.</p>","PeriodicalId":12138,"journal":{"name":"Food additives and contaminants","volume":" ","pages":"1345-52"},"PeriodicalIF":0.0000,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/02652030701458097","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food additives and contaminants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/02652030701458097","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
Aminoglycoside antibiotics are commonly used to treat bacterial infections in human and veterinary practice. Owing to their toxicity, the European Community has established maximum residue limits (MRL) in foodstuffs of animal origin (EEC No 2377/90). In the present study, the performance of two new enzyme immunoassays (EIA), I'screen Gentamicin and I'screen Neomycin, for the quantitative detection of the aminoglycosides, gentamicin and neomycin, in milk and tissue are described. Validation of these EIAs has been performed in accordance to the criteria of European Decision 657/2002. Assays sensitivity at the MRLs was 95% for milk samples and 100% for tissue samples, while specificity was 100% at 33 and 25% of the MRLs for milk and tissues, respectively. The performance of these EIAs indicates that they can be used as easy screening methods for the analysis of aminoglycosides in milk and tissue samples.
氨基糖苷类抗生素在人类和兽医实践中通常用于治疗细菌感染。由于它们的毒性，欧洲共同体已经在动物源性食品中建立了最大残留限量(MRL) (EEC No 2377/90)。本文介绍了两种新的酶免疫分析法(EIA)，即I'screen庆大霉素和I'screen新霉素，用于牛奶和组织中氨基糖苷类庆大霉素和新霉素的定量检测。这些环评的验证已按照欧洲第657/2002号决议的标准进行。该检测方法对牛奶样品的MRLs的敏感性为95%，对组织样品的MRLs的敏感性为100%，而对牛奶和组织样品的特异性分别为33%和25%。结果表明，该方法可作为牛奶和组织样品中氨基糖苷类化合物的简便筛选方法。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
