Validation of an analytical method for the determination of carbadox and olaquindox in feedstuff by liquid chromatography coupled to UV and/or diode array detection.

Food additives and contaminants Pub Date : 2007-11-01 DOI:10.1080/02652030701405544

G Gizzi, U Vincent, C von Holst, J de Jong, C Genouel

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引用次数: 13

Abstract

The performance characteristics of an analytical method based on high-performance liquid chromatography (HPLC) for the detection of the banned growth promoters, carbadox and olaquindox, in feedstuff were determined via a collaborative study. The relative standard deviation of repeatability (RSDr) ranged 1.1-5.5% for carbadox and 2.5-6.2% for olaquindox. The relative standard deviation of reproducibility (RSDR) ranged 6.4-10.7% for carbadox and 12.8-20.0% for olaquindox. In all cases, the HORRAT values were equal or below the critical value of 1.5. Moreover, trueness in all cases was between the acceptance limits of 80 and 110%. Consequently, it was concluded that the method is suitable for quantitative evaluation. The method was also qualitatively assessed in terms of correct identification of the target analytes by examination of the UV spectrum when the more specific diode array detector was coupled to HPLC. In all cases, the percentage of correct identifications was >or=94% for olaquindox and carbadox, while the percentage of false negatives was

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液相色谱耦合紫外和/或二极管阵列检测法测定饲料中卡巴多和喹啉的分析方法的验证。

通过合作研究，建立了高效液相色谱法检测饲料中禁用生长促进剂卡瓦多和喹啉的性能特征。卡巴多的重复性相对标准偏差(RSDr)为1.1 ~ 5.5%，喹啉的重复性相对标准偏差为2.5 ~ 6.2%。卡巴多的重复性相对标准偏差(RSDR)为6.4 ~ 10.7%，喹啉的重复性相对标准偏差为12.8 ~ 20.0%。在所有情况下，HORRAT值都等于或低于临界值1.5。此外，所有情况下的正确率都在80%到110%的可接受范围之间。结果表明，该方法适用于定量评价。当更具体的二极管阵列检测器与高效液相色谱相结合时，通过检查紫外光谱对目标分析物的正确识别，也对该方法进行了定性评估。在所有病例中，对邻喹啉和卡瓦多的正确鉴定百分比>或=94%，而假阴性百分比为

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Food additives and contaminants

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