{"title":"Development of a novel genosensor based on ferrocenyl oligonucleotides.","authors":"Kosuke Mukumoto, Takahiko Nojima, Nobuaki Furuno, Shigeori Takenaka","doi":"10.1093/nass/3.1.43","DOIUrl":null,"url":null,"abstract":"<p><p>Two types of ferrocenyl oligonucleotides, Fc1-ODN and Fc2-ODN, carrying a 5'-ATT GCT CAG GGG TAA GGT CAT TAG TTG GAA-3' sequence were allowed to hybridize with the DNA probe carrying a complementary sequence immobilized on the gold electrode to give rise to a redox signal deriving from their ferrocenyl moieties. When a mixture of these oligonucleotides in a proper ratio was used as a model of DNA samples, two redox peaks were observed in one differential pulse voltammogram. The peak currents varied depending on the initial ratio of the two oligonucleotides, suggesting a possibility that this technique may be applied to electrochemical monitoring of gene expression by, for example, the electrochemical differential hybridization, EDH.</p>","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"43-4"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.43","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids research. Supplement (2001)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/3.1.43","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Two types of ferrocenyl oligonucleotides, Fc1-ODN and Fc2-ODN, carrying a 5'-ATT GCT CAG GGG TAA GGT CAT TAG TTG GAA-3' sequence were allowed to hybridize with the DNA probe carrying a complementary sequence immobilized on the gold electrode to give rise to a redox signal deriving from their ferrocenyl moieties. When a mixture of these oligonucleotides in a proper ratio was used as a model of DNA samples, two redox peaks were observed in one differential pulse voltammogram. The peak currents varied depending on the initial ratio of the two oligonucleotides, suggesting a possibility that this technique may be applied to electrochemical monitoring of gene expression by, for example, the electrochemical differential hybridization, EDH.
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