Differential transcriptional regulation of two distinct S-adenosylmethionine synthetase genes (SAM1 and SAM2) of Saccharomyces cerevisiae.

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI:10.1093/nass/3.1.303

Tsutomu Kodaki, Shinji Tsuji, Naoko Otani, Daihei Yamamoto, Kota Sreenivasa Rao, Seiya Watanabe, Masahiro Tsukatsune, Keisuke Makino

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引用次数: 13

Abstract

Expression of a number of genes encoding enzymes involved in phospholipid biosynthesis in yeast Saccharomyces cerevisiae is known to be repressed on the addition of myo-inositol and choline to the culture medium (inositol-choline regulation). All genes subject to this inositol-choline regulation have an octamer sequence 5'-CATRTGAA-3' in their upstream regions and those octamer sequences play an important role in this regulation. To confirm the role of the octamer sequence further, we studied the transcriptional regulation of two distinct S-adenosylmethionine synthetase genes (SAM1 and SAM2) of S. cerevisiae. Quantitative RT-PCR analysis showed that only the SAM2 gene was subject to the inositol-choline regulation, consistent with the fact that only the SAM2 gene has two octamer sequences in its upstream region. Furthermore, functional promoter analysis revealed that the proximal octamer sequence of the SAM2 gene has an essential role for this regulation.

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酿酒酵母两种不同s -腺苷蛋氨酸合成酶基因(SAM1和SAM2)的差异转录调控

在酵母中，一些编码磷脂生物合成酶的基因的表达在培养基中添加肌醇和胆碱时被抑制(肌醇-胆碱调节)。所有受肌醇-胆碱调控的基因在其上游区域都有一个八聚体序列5'-CATRTGAA-3'，这些八聚体序列在这种调控中起着重要作用。为了进一步确认八聚体序列的作用，我们研究了酿酒酵母两个不同的s -腺苷蛋氨酸合成酶基因(SAM1和SAM2)的转录调控。定量RT-PCR分析表明，只有SAM2基因受肌醇-胆碱调控，这与SAM2基因上游区域只有两个八聚体序列的事实相一致。此外，功能启动子分析显示SAM2基因的近端八聚体序列在这一调控中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nucleic acids research. Supplement (2001)

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