{"title":"Investigation of physical and physiological properties of 4'-thioribonucleotide (4'-thioRNA).","authors":"Shuichi Hoshika, Naonori Inoue, Noriaki Minakawa, Akira Matsuda","doi":"10.1093/nass/3.1.209","DOIUrl":null,"url":null,"abstract":"<p><p>An efficient and practical synthesis of 4'-thioribonucleosides was accomplished via the Pummerer reaction. The resulting 4'-thioribonucleosides were converted into the corresponding phosphoramidite units, and 4'-thioribonucleic acids (4'-thioRNAs) of 15 mer were synthesized on a DNA synthesizer using a controlled pore glass (CPG) with 4'-thiouridine unit. The thermal stability of 4'-thioRNA:RNA duplex was higher than that of RNA:RNA duplex. Moreover, the thermal stability of 4'-thioRNA:4'-thioRNA duplex was the highest and stabilized 30 degrees C or more compared with RNA:RNA duplex (>99 degrees C vs 66 degrees C). Structural analysis by CD spectra indicates that 4'-thioRNA:4'-thioRNA duplex and 4'-thioRNA:RNA duplex adapt A-form conformation as well as RNA:RNA duplex.</p>","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"209-10"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.209","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids research. Supplement (2001)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/3.1.209","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
An efficient and practical synthesis of 4'-thioribonucleosides was accomplished via the Pummerer reaction. The resulting 4'-thioribonucleosides were converted into the corresponding phosphoramidite units, and 4'-thioribonucleic acids (4'-thioRNAs) of 15 mer were synthesized on a DNA synthesizer using a controlled pore glass (CPG) with 4'-thiouridine unit. The thermal stability of 4'-thioRNA:RNA duplex was higher than that of RNA:RNA duplex. Moreover, the thermal stability of 4'-thioRNA:4'-thioRNA duplex was the highest and stabilized 30 degrees C or more compared with RNA:RNA duplex (>99 degrees C vs 66 degrees C). Structural analysis by CD spectra indicates that 4'-thioRNA:4'-thioRNA duplex and 4'-thioRNA:RNA duplex adapt A-form conformation as well as RNA:RNA duplex.
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