{"title":"The electron-microscopic stucture and chemical composition of the isolated sarcolemma of the rat skeletal muscle cell","authors":"Tetsuro Kono , Fumiko Kakuma, Mitsunobu Homma, Shoichi Fukuda","doi":"10.1016/0926-6577(64)90163-9","DOIUrl":null,"url":null,"abstract":"<div><p>Purified tubular membranes prepared by extracting rat skeletal muscle cell fragments with buffered 0.4 M LiBr solution have been shown by electron microscopy to retain the major three layers (outer network of collagen fibrils, middle basement membrane, and inner plasma membrane) of the sarcolemma of the intact muscle cell. A chloroformmethanol mixture destroyed the plasma membrane, thereby extracting steroid, glyceride, phosphatide, protein and carbohydrate. A collagenase (EC 3.4.4.19) preparation appeared to digest not only the collagen network, but also the basement membrane. The lipid extractio or collagenase treatment did not destroy the tubular structure of the isolated membrane, but successive application of both treatments yielded only an amorphous precipitate.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 1","pages":"Pages 155-176"},"PeriodicalIF":0.0000,"publicationDate":"1964-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90163-9","citationCount":"35","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764901639","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 35
Abstract
Purified tubular membranes prepared by extracting rat skeletal muscle cell fragments with buffered 0.4 M LiBr solution have been shown by electron microscopy to retain the major three layers (outer network of collagen fibrils, middle basement membrane, and inner plasma membrane) of the sarcolemma of the intact muscle cell. A chloroformmethanol mixture destroyed the plasma membrane, thereby extracting steroid, glyceride, phosphatide, protein and carbohydrate. A collagenase (EC 3.4.4.19) preparation appeared to digest not only the collagen network, but also the basement membrane. The lipid extractio or collagenase treatment did not destroy the tubular structure of the isolated membrane, but successive application of both treatments yielded only an amorphous precipitate.
电镜显示,用缓冲的0.4 M LiBr溶液提取大鼠骨骼肌细胞片段制备的纯化管状膜保留了完整肌细胞肌膜的主要三层(胶原原纤维外网、中基底膜和内质膜)。氯仿甲醇混合物破坏质膜,从而提取类固醇、甘油、磷脂、蛋白质和碳水化合物。胶原酶(EC 3.4.4.19)不仅能消化胶原网络,还能消化基底膜。脂质提取或胶原酶处理没有破坏分离膜的管状结构,但连续应用这两种处理只产生无定形沉淀。