Hoa Thi Tran, Giang Thu Nguyen, Hong Ha Thi Nguyen, Huyen Thi Tran, Quang Hong Tran, Quang Ho Tran, Ngoc Thi Ninh, Phat Tien Do, Ha Hoang Chu, Ngoc Bich Pham
{"title":"Isolation and Cytotoxic Potency of Endophytic Fungi Associated with <i>Dysosma difformis</i>, a Study for the Novel Resources of Podophyllotoxin.","authors":"Hoa Thi Tran, Giang Thu Nguyen, Hong Ha Thi Nguyen, Huyen Thi Tran, Quang Hong Tran, Quang Ho Tran, Ngoc Thi Ninh, Phat Tien Do, Ha Hoang Chu, Ngoc Bich Pham","doi":"10.1080/12298093.2022.2126166","DOIUrl":null,"url":null,"abstract":"<p><p>Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes associated with <i>Dysosma difformis</i>-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. <i>Fusarium</i> was found the most prevalent genus with a colonization frequency of 11.11%, followed by <i>Trametes</i> (9.26%) and <i>Penicillium</i> (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specific tissues and habitats. Cytotoxic activity of endophytic fungal extracts was investigated on cancer cell lines such as SK-LU-1, HL-60, and HepG2, demonstrating strong anti-cancer activity of six isolates belonging to <i>Penicillium, Trametes, Purpureocillium, Aspergillus,</i> and <i>Ganoderma</i> with IC<sub>50</sub> value of lower than 10 <i>µ</i>g/mL. The presence of podophyllotoxin was indicated in <i>Penicillium, Trametes, Aspergillus</i> and for the first time in <i>Purpureocillium</i> and <i>Ganoderma via</i> high-performance liquid chromatography, which implied them as a potential source of this anti-cancer compound.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"50 5","pages":"389-398"},"PeriodicalIF":1.6000,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/51/19/TMYB_50_2126166.PMC9645267.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2022.2126166","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 1
Abstract
Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes associated with Dysosma difformis-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. Fusarium was found the most prevalent genus with a colonization frequency of 11.11%, followed by Trametes (9.26%) and Penicillium (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specific tissues and habitats. Cytotoxic activity of endophytic fungal extracts was investigated on cancer cell lines such as SK-LU-1, HL-60, and HepG2, demonstrating strong anti-cancer activity of six isolates belonging to Penicillium, Trametes, Purpureocillium, Aspergillus, and Ganoderma with IC50 value of lower than 10 µg/mL. The presence of podophyllotoxin was indicated in Penicillium, Trametes, Aspergillus and for the first time in Purpureocillium and Ganoderma via high-performance liquid chromatography, which implied them as a potential source of this anti-cancer compound.
期刊介绍:
Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.