Effects of propofol and dexmedetomidine on carnitine metabolism in normal human bronchial epithelial cells.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnic & Histochemistry Pub Date : 2023-01-01 Epub Date: 2022-08-05 DOI:10.1080/10520295.2022.2107239
Evren Büyükfırat, Ismail Koyuncu, Mahmut Alp Karahan, Ataman Gönel, Orhan Binici, Erdoğan Duran, Nuray Altay
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引用次数: 0

Abstract

Propofol and dexmedetomidine (DEX) are widely used for anesthesia and sedation. We investigated the effects of propofol and DEX separately and in combination on the metabolic profile of carnitine in cultured normal human bronchial epithelial cells (BEAS-2B). Cells of the propofol group were cultured with 2 µg/ml propofol in RPMI-1640 medium. Cells of the DEX group were cultured with 0.2 ng/m DEX in RPMI-1640 medium. Cells of the propofol + DEX group were cultured with 2 μg/ml propofol + 0.2 ng/ml DEX in RPMI-1640 medium. The control group was untreated. Cells were incubated for 3 h following treatments. The effects of the drugs on cell viability were assessed using the MTT method and by microscopic examination following staining with acridine orange/ethidium bromide. The effects of drugs on carnitine, acetyl carnitine and 25 acylcarnitine derivative profiles were analyzed using liquid chromatography-tandem mass spectrophotometry. Neither propofol nor DEX affected cell viability. Administration of propofol, DEX or propofol + DEX to BEAS-2B cells caused no significant change in the concentrations of carnitine and acylcarnitine derivatives compared to the control group. We found that propofol and DEX exhibit no negative effects on the carnitine metabolism by BEAS-2B cells in vitro at clinically relevant concentrations. Our findings establish a baseline for clinical studies of the effects of propofol and DEX on carnitine metabolism.

异丙酚和右美托咪定对正常人支气管上皮细胞肉碱代谢的影响。
异丙酚和右美托咪定(DEX)广泛用于麻醉和镇静。我们研究了异丙酚和DEX单独和联合使用对培养的正常人支气管上皮细胞(BEAS-2B)中肉碱代谢谱的影响。异丙酚组细胞用2µg/ml异丙酚在RPMI-1640培养基中培养。DEX组细胞以0.2 ng/m DEX在RPMI-1640培养基中培养。异丙酚+ DEX组细胞用2 μg/ml异丙酚+ 0.2 ng/ml DEX在RPMI-1640培养基中培养。对照组不进行治疗。处理后,细胞孵育3小时。采用MTT法和吖啶橙/溴化乙锭染色镜检评估药物对细胞活力的影响。采用液相色谱-串联质谱法分析了药物对肉毒碱、乙酰肉毒碱和25个酰基肉毒碱衍生物谱的影响。异丙酚和DEX均不影响细胞活力。与对照组相比,异丙酚、DEX或异丙酚+ DEX给BEAS-2B细胞对肉毒碱和酰基肉毒碱衍生物的浓度没有显著变化。我们发现异丙酚和DEX在临床相关浓度下对BEAS-2B细胞体外肉毒碱代谢无负面影响。我们的研究结果为临床研究异丙酚和右咪唑对肉毒碱代谢的影响奠定了基础。
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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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