Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles.

Journal of applied oral science : revista FOB Pub Date : 2022-11-07 eCollection Date: 2022-01-01 DOI:10.1590/1678-7757-2022-0158

Miyuki Sakuma, Kouji Ohta, Shohei Fukada, Misaki Akagi, Hiroki Kato, Yoko Ishida, Takako Naruse, Masaaki Takechi, Hideo Shigeishi, Hiromi Nishi, Tomonao Aikawa

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Abstract

Objective: Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the carcinoembryonic antigen family. Although its expression has been found in chronic oral inflammatory epithelium, this study aimed to know whether CEACAM1 in oral keratinocytes participates in host immune response against Candida albicans .

Methodology: We investigated CEACAM1 expression in oral keratinocytes induced by C. albicans as well as by Candida cell wall component β-glucan particles (β-GPs). Furthermore, the effects of CEACAM1 on β-GPs-induced heme oxygenase-1 (HO-1) expression and its related signals were examined.

Results: Fluorescence staining showed CEACAM1 expression in oral keratinocytes (RT7) cells, whereas quantitative reverse transcription (RT)-PCR indicated that both live and heat-killed C. albicans increased CEACAM1 mRNA expression in RT7 cells. Examinations using quantitative RT-PCR and western blotting indicated that CEACAM1 expression was also increased by β-GPs derived from C. albicans . Specific siRNA for CEACAM1 decreased HO-1 expression induced by β-GPs from C. albicans as well as the budding yeast microorganism Saccharomyces cerevisiae . Moreover, knockdown of CEACAM1 decreased β-GPs-induced ROS activity in the early phase and translocation of Nrf2 into the nucleus.

Conclusion: CEACAM1 in oral keratinocytes may have a critical role in regulation of HO-1 for host immune defense during Candida infection.

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口腔角质形成细胞CEACAM1对念珠菌β-葡聚糖颗粒诱导HO-1表达的影响。

目的:癌胚抗原相关细胞粘附分子1 (CEACAM1)是癌胚抗原家族的一员。虽然在口腔慢性炎症上皮中已发现CEACAM1的表达，但本研究旨在了解CEACAM1在口腔角化细胞中是否参与宿主对白色念珠菌的免疫应答。方法:研究CEACAM1在白色念珠菌诱导的口腔角化细胞和念珠菌细胞壁成分β-葡聚糖颗粒(β-GPs)诱导的口腔角化细胞中的表达。此外，我们还检测了CEACAM1对β- gps诱导的血红素氧合酶-1 (HO-1)表达及其相关信号的影响。结果:荧光染色显示CEACAM1在口腔角质形成细胞(RT7)中表达，而定量反转录(RT -PCR)显示活的和热灭活的白色念珠菌均增加了RT7细胞中CEACAM1 mRNA的表达。定量RT-PCR和western blotting检测显示，来自白色念珠菌的β-GPs也增加了CEACAM1的表达。CEACAM1特异性siRNA可降低白色念珠菌和酿酒酵母芽殖微生物β-GPs诱导的HO-1表达。此外，CEACAM1的敲低降低了β- gps诱导的早期ROS活性和Nrf2向细胞核的易位。结论:口腔角化细胞中的CEACAM1可能在假丝酵母感染时HO-1对宿主免疫防御的调控中起关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of applied oral science : revista FOB

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