Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons.

IF 1.2 4区医学 Q3 PATHOLOGY

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-08-06 DOI:10.1007/s00795-022-00330-z

Noriko Hiramatsu, Naoki Yamamoto, Mahito Ohkuma, Noriaki Nagai, Ei-Ichi Miyachi, Kumiko Yamatsuta, Kazuyoshi Imaizumi

引用次数: 0

Abstract

When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.

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在小鼠所有体细胞中表达GFP并分化为功能性视网膜神经元的虹膜来源诱导多能干细胞。

当诱导多能干细胞(iPSCs)产生再生组织时，有必要对移植细胞进行跟踪和鉴定。荧光标记的iPSCs合成一种易于追踪的荧光物质。然而，表达的蛋白不应影响原始基因组序列或多能性。为了解决这个问题，我们创建了一个细胞工具，用于多能干细胞的基础研究。将来自GFP荧光表达小鼠(GFP- dba /2小鼠)的虹膜组织来源细胞重新编程，生成GFP小鼠虹膜来源的iPSCs (m -虹膜GFP iPSCs)。M-iris GFP诱导多能干细胞表达了诱导多能干细胞特征的细胞标记物，并在分化为三胚层时表现出多能性。此外，当表达GFP时，细胞分化为功能恢复蛋白和钙结合蛋白阳性细胞。因此，该细胞系将为未来iPSCs的研究提供便利。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Molecular Morphology 医学-病理学

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

>12 weeks

期刊介绍： Medical Molecular Morphology is an international forum for researchers in both basic and clinical medicine to present and discuss new research on the structural mechanisms and the processes of health and disease at the molecular level. The structures of molecules, organelles, cells, tissues, and organs determine their normal function. Disease is thus best understood in terms of structural changes in these different levels of biological organization, especially in molecules and molecular interactions as well as the cellular localization of chemical components. Medical Molecular Morphology welcomes articles on basic or clinical research in the fields of cell biology, molecular biology, and medical, veterinary, and dental sciences using techniques for structural research such as electron microscopy, confocal laser scanning microscopy, enzyme histochemistry, immunohistochemistry, radioautography, X-ray microanalysis, and in situ hybridization. Manuscripts submitted for publication must contain a statement to the effect that all human studies have been reviewed by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. It should also be stated clearly in the text that all persons gave their informed consent prior to their inclusion in the study. Details that might disclose the identity of the subjects under study should be omitted.