Effect of H2O2 on Na,K-ATPase.

IF 2.9 4区 生物学 Q2 BIOPHYSICS
Gvantsa Chkadua, Eka Nozadze, Leila Tsakadze, Lia Shioshvili, Nana Arutinova, Marine Leladze, Sopio Dzneladze, Maia Javakhishvili
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引用次数: 4

Abstract

Na,K-ATPase is a member of the P-type ATPase family, which transforms the energy of ATP to the transmembrane Na/K gradient that is used to create membrane potential, support the excitability of neurons and myocytes, control pH, and transport substances. The regulation of the Na,K-ATPase function by physiological regulators also comprises a central role in the adaptation of organisms to different conditions. H2O2 is one of the main signaling molecules in redox metabolism and plays important function in cellular physiology. H2O2 also regulates signaling pathways via the specific oxidation of proteins harboring redox-sensitive moieties, like metal centers or cysteine residues, which control their activity. The Na,K-ATPase is redox-sensitive with an "optimal redox potential range," where the reactive oxygen species (ROS), levels beyond this "optimal range" are responsible for enzyme inhibition. Thus reactive oxygen species manifest a hermetic effect, which is expressed by biphasic action; stimulation by low doses and inhibition by high doses. This study was aimed to reveal redox-sensitivity of brain synaptic membrane fractions Na,K-ATPase via H2O2 effects. Different concentrations of H2O2 change the kinetic parameters of the enzyme system for MgATP complex, Na+, and K+ differently. Moreover, H2O2 changes p-chloromercuribenzoic acids (PCMB) affinity. H2O2 targets thiols of the Na,K-ATPase - low and high concentrations of H2O2 change the oxidative state of thiolate (S-) from Cys differently, resulting in the corresponding activation or inhibition of the enzyme. Targeting thiols of the Na,K-ATPase tunes the activity of the Na,K-ATPase to the cellular demands and sustains the enzyme activity at the "optimal" level.

Abstract Image

H2O2对Na、k - atp酶的影响。
Na,K-ATP酶是p型ATP酶家族的一员,它将ATP的能量转化为跨膜的Na/K梯度,用于产生膜电位,支持神经元和肌细胞的兴奋性,控制pH和转运物质。生理调节剂对Na, k - atp酶功能的调节也在生物体适应不同条件中起着核心作用。H2O2是氧化还原代谢的主要信号分子之一,在细胞生理中起着重要作用。H2O2还通过对含有氧化还原敏感部分(如金属中心或半胱氨酸残基)的蛋白质的特定氧化来调节信号通路,从而控制其活性。Na, k - atp酶对氧化还原敏感,具有“最佳氧化还原电位范围”,其中超过该“最佳范围”的活性氧(ROS)水平负责酶抑制。因此,活性氧表现出一种封闭效应,这种封闭效应表现为双相作用;低剂量刺激和高剂量抑制。本研究旨在通过H2O2效应揭示脑突触膜组分Na, k - atp酶的氧化还原敏感性。不同浓度的H2O2对MgATP复合物、Na+和K+酶系统的动力学参数有不同的影响。H2O2还改变了对氯基苯甲酸(PCMB)的亲和力。H2O2以Na、k - atp酶的硫醇为目标,低浓度和高浓度的H2O2不同程度地改变了来自Cys的硫酸盐(S-)的氧化状态,从而导致相应的酶活化或抑制。靶向Na, k - atp酶的硫醇调节Na, k - atp酶的活性以满足细胞的需要,并将酶活性维持在“最佳”水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
22
审稿时长
6-12 weeks
期刊介绍: The Journal of Bioenergetics and Biomembranes is an international journal devoted to the publication of original research that contributes to fundamental knowledge in the areas of bioenergetics, biomembranes, and transport, including oxidative phosphorylation, photosynthesis, muscle contraction, as well as cellular and systemic metabolism. The timely research in this international journal benefits biophysicists, membrane biologists, cell biologists, biochemists, molecular biologists, physiologists, endocrinologists, and bio-organic chemists.
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