Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2022-07-25 DOI:10.1186/s12860-022-00435-4

Nesa Janamo Berenjabad, Vahid Nejati, Jafar Rezaie

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引用次数: 3

Abstract

Background: Many attempts are used to discover mechanisms driving impaired angiogenesis in age-related diseases. Angiogenesis is highly regulated by different signaling pathways. Here, we investigated the angiogenesis potential of human endothelial cells (ECs) upon exposure to hydrogen peroxide (H₂O₂), a cellular senescent factor.

Results: Data showed that the wound healing rate of HUVECs decreased upon incubation with H₂O₂ (P < 0.05). LOX activity and NO production were decreased in H₂O₂ treated cells (P < 0.05). Expression of miR-126 and VEGFR-2 up-regulated, while expression of miR-373 and HSP-70 up = regulated in H₂O₂ -induced cells (P < 0.05). In addition, we found that protein levels of p-Akt-1, VCAM-1, MMP-9, and IL-6 decreased in treated cells (P < 0.05).

Conclusions: Our data showed that H₂O₂ reduced the angiogenic response of HUVECs in vitro, which may be due to impairment of the VEGFR-2 signaling pathway.

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过氧化氢诱导衰老后，人内皮细胞的血管生成能力下降:可能与vegfr-2/akt-1信号通路有关。

背景:许多研究试图发现衰老相关疾病中血管生成受损的机制。血管生成受不同信号通路的高度调控。在这里，我们研究了暴露于过氧化氢(H2O2)(一种细胞衰老因子)后人内皮细胞(ECs)的血管生成潜力。结果:数据显示H2O2 (p2o2)处理细胞(p2o2)诱导的HUVECs伤口愈合速率降低。结论:我们的数据显示H2O2降低了HUVECs体外血管生成反应，这可能与VEGFR-2信号通路受损有关。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.