Key factors to establish the ovalbumin-induced atopic dermatitis minipig model: age and body weight.

IF 2.7 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Laboratory Animal Research Pub Date : 2022-10-20 DOI:10.1186/s42826-022-00141-4

Young Kyu Kim, JuKyung Lee, Hyeon-Young Kim, Sung-Hwan Kim, Jeong Ho Hwang, Han Na Suh

{"title":"Key factors to establish the ovalbumin-induced atopic dermatitis minipig model: age and body weight.","authors":"Young Kyu Kim, JuKyung Lee, Hyeon-Young Kim, Sung-Hwan Kim, Jeong Ho Hwang, Han Na Suh","doi":"10.1186/s42826-022-00141-4","DOIUrl":null,"url":null,"abstract":"Background: Given its similar structure and immune response to the human skin, porcine is a good model for dermal studies. Here, we sensitized ovalbumin (Ova) on minipig back skin for 2-4 weeks to induce chronic atopic dermatitis (AD).Results: Gross observation, serum cytokine level, epidermal thickness, and epidermal integrity did not change after 4 weeks of Ova induction compared with the control, indicating AD modeling failure. Only the neutrophils in the blood and macrophages in bronchoalveolar lavage fluid changed slightly until 3 or 2 weeks after Ova sensitization, respectively. The successful and failed Ova-induced AD minipig models only differ in age and body weight of the minipigs. The minipigs, 12 months old with a 30-kg median weight, had a two-fold thicker dermis than minipigs 8-10 months old, with an 18.97-kg median weight, resulting in impaired Ova permeability and immune response.Conclusion: Age and body weight are key factors that should be considered when developing an Ova-induced AD minipig model.","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585725/pdf/","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laboratory Animal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s42826-022-00141-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 1

Abstract

Background: Given its similar structure and immune response to the human skin, porcine is a good model for dermal studies. Here, we sensitized ovalbumin (Ova) on minipig back skin for 2-4 weeks to induce chronic atopic dermatitis (AD).

Results: Gross observation, serum cytokine level, epidermal thickness, and epidermal integrity did not change after 4 weeks of Ova induction compared with the control, indicating AD modeling failure. Only the neutrophils in the blood and macrophages in bronchoalveolar lavage fluid changed slightly until 3 or 2 weeks after Ova sensitization, respectively. The successful and failed Ova-induced AD minipig models only differ in age and body weight of the minipigs. The minipigs, 12 months old with a 30-kg median weight, had a two-fold thicker dermis than minipigs 8-10 months old, with an 18.97-kg median weight, resulting in impaired Ova permeability and immune response.

Conclusion: Age and body weight are key factors that should be considered when developing an Ova-induced AD minipig model.

Abstract Image

查看原文本刊更多论文

建立卵清蛋白致特应性皮炎迷你猪模型的关键因素:年龄和体重。

背景:猪具有与人类皮肤相似的结构和免疫反应，是皮肤研究的良好模型。在这里，我们在迷你猪背部皮肤上致敏卵白蛋白(Ova) 2-4周，以诱导慢性特应性皮炎(AD)。结果:肉眼观察，与对照组相比，卵细胞诱导4周后血清细胞因子水平、表皮厚度和表皮完整性均未发生变化，提示AD造模失败。只有血液中的中性粒细胞和支气管肺泡灌洗液中的巨噬细胞分别在卵致敏后3周和2周略有变化。成功和失败的ova诱导AD迷你猪模型仅在猪的年龄和体重上存在差异。12月龄中位体重为30 kg的迷你猪真皮厚度是8-10月龄中位体重为18.97 kg的迷你猪的2倍，导致卵子通透性和免疫反应受损。结论:年龄和体重是建立ova诱导的AD迷你猪模型时应考虑的关键因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Laboratory Animal Research Multiple-

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

8 weeks