Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2022-07-25 DOI:10.1021/acs.nanolett.2c02259

Min Han, Hongru Yang, Xiangdong Lu, Yuming Li, Zihao Liu, Feng Li, Zehan Shang, Xiaofeng Wang, Xuze Li, Junliang Li, Hong Liu* and Tao Xin*,

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引用次数: 37

Abstract

Exosomes derived from mesenchymal stem cells (MSCs) have been proven to exhibit great potentials in spinal cord injury (SCI) therapy. However, conventional two-dimensional (2D) culture will inevitably lead to the loss of stemness of MSCs, which substantially limits the therapeutic potency of MSCs exosomes (2D-Exo). Exosomes derived from three-dimensional culture (3D-Exo) possess higher therapeutic efficiency which have wide applications in spinal cord therapy. Typically, conventional exosome therapy that relies on local repeated injection results in secondary injury and low efficiency. It is urgent to develop a more reliable, convenient, and effective exosome delivery method to achieve constant in situ exosomes release. Herein, we proposed a controlled 3D-exohydrogel hybrid microneedle array patch to achieve SCI repair in situ. Our studies suggested that MSCs with 3D-culturing could maintain their stemness, and consequently, 3D-Exo effectively reduced SCI-induced inflammation and glial scarring. Thus, it is a promising therapeutic strategy for the treatment of SCI.

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三维培养msc衍生外泌体-水凝胶混合微针阵列贴片用于脊髓修复

来自间充质干细胞(MSCs)的外泌体已被证明在脊髓损伤(SCI)治疗中具有巨大的潜力。然而，传统的二维(2D)培养将不可避免地导致MSCs的干性丧失，这极大地限制了MSCs外泌体(2D- exo)的治疗效力。三维培养外泌体(3D-Exo)具有较高的治疗效果，在脊髓治疗中有广泛的应用。通常，传统的外泌体治疗依赖于局部重复注射，导致继发性损伤和低效率。迫切需要开发一种更可靠、方便、有效的外泌体递送方法来实现外泌体的恒定原位释放。在此，我们提出了一种受控的3d -外水凝胶混合微针阵列贴片来实现SCI的原位修复。我们的研究表明，3d培养的MSCs可以保持其干性，因此3D-Exo有效地减少了sci诱导的炎症和胶质瘢痕。因此，它是一种很有前途的治疗脊髓损伤的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.