Transmembrane Water Transport and Intracellular Ice Formation of Human Umbilical Vein Endothelial Cells During Freezing.

IF 1.2 4区生物学 Q4 CELL BIOLOGY

Biopreservation and Biobanking Pub Date : 2022-08-01 DOI:10.1089/bio.2022.0111

Yu Huang, Ying Dong, Brian Gao, Ruidong Ma, Frank Liu Gao, Lingxiao Shen

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引用次数: 1

Abstract

Long-term cryopreservation of human umbilical vein endothelial cells (HUVECs) is important and beneficial for a variety of biomedical research and applications. In this study, we investigated HUVEC's cryobiological characteristics and parameters that are indispensable for predicting and determining an optimal cooling rate to prevent lethal intracellular ice formation (IIF) and severe cell dehydration during the cryopreservation processes. The parameters include cell membrane hydraulic conductivity (i.e., cell membrane water permeability), L_p, cell membrane water permeability activation energy, E_lp, and osmotically inactive volume of a cell V_b. Cryomicroscopy was used to study the IIF phenomena and cell volume excursion at various cooling rates, 1, 10, and 20°C/min, respectively, based on which the cryobiological parameters were determined using biophysical and mathematical models. Results from this research work laid an important cryobiological foundation for the optimization of HUVEC's cryopreservation conditions.

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人脐静脉内皮细胞冷冻过程中水的跨膜转运和细胞内冰的形成。

人脐静脉内皮细胞(HUVECs)的长期低温保存对多种生物医学研究和应用具有重要意义。在这项研究中，我们研究了HUVEC的低温生物学特性和参数，这些特性和参数对于预测和确定在低温保存过程中防止致命细胞内冰形成(IIF)和严重细胞脱水的最佳冷却速率是必不可少的。参数包括细胞膜的水导率(即细胞膜的水透性)、Lp、细胞膜的水透性活化能、Elp、细胞的渗透失活体积Vb。采用低温显微镜研究不同冷却速率(1、10和20°C/min)下的IIF现象和细胞体积偏移，并在此基础上利用生物物理和数学模型确定低温生物学参数。本研究结果为优化HUVEC的低温保存条件奠定了重要的低温生物学基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biopreservation and Biobanking CELL BIOLOGY-MEDICAL LABORATORY TECHNOLOGY

CiteScore

3.30

自引率

12.50%

发文量

114

审稿时长

6-12 weeks

期刊介绍： Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.