Anti-Toxoplasma Effects of Dracocephalum Polychaetum Essential Oil.

Q3 Immunology and Microbiology

Interdisciplinary Perspectives on Infectious Diseases Pub Date : 2022-07-16 eCollection Date: 2022-01-01 DOI:10.1155/2022/6091834

Faham Khamesipour, Amirhossein Pourmohammad, Mohsen Jafarian-Dehkordi

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Abstract

Background: Toxoplasma gondii is a common parasitic disease with a cosmopolitan prevalence, causing severe health problems. Although chemotherapy for toxoplasmosis is readily available, most have side effects.

Objectives: This study assesses the Dracocephalum polychaetum essential oil against T. gondii activity. In vitro, the anti-Toxoplasma effects of D. polychaetum essential oils with different concentrations were evaluated.

Methods: In the present study, T. gondii RH strain tachyzoites were exposed to D. polychaetum essential oil, and their viability effect on the parasite was evaluated. The viability test of tachyzoites was performed by using the staining method trypan blue in vitro. The inhibitory effect of D. polychaetum extract on T. gondii RH strain tachyzoites in the Vero cell line was evaluated.

Results: D. polychaetum has valuable efficacy in vitro, outperforming pyrimethamine and sulfadiazine at 30 and 90 minutes after exposure (p < 0.05). D. polychaetum essential oil showed anti-Toxoplasma activity in the cell line (IC50: 241.7 μg/mL). After T. gondii-infected Vero cells had been incubated with different concentrations of the D. polychaetum essential oil, their viability decreased in a dose-dependent manner.

Conclusions: In conclusion, D. polychaetum extract as an herbal medicine might be a valuable alternative to routine chemotherapy for toxoplasmosis.

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龙脑香精油的抗弓形虫作用

背景：弓形虫是一种常见的寄生虫病，在全球流行，造成严重的健康问题。虽然弓形虫病的化疗很容易获得，但大多数都有副作用：本研究评估了龙脑香精油对弓形虫的活性。在体外，评估了不同浓度的多毛虫精油的抗弓形虫效果：在本研究中，将弓形虫 RH 菌株的蝌蚪暴露于多毛象甲虫精油中，并评估其对寄生虫的活力影响。在体外使用胰蓝染色法检测寄生虫的存活率。评估了 D. polychaetum 提取物在 Vero 细胞系中对 T. gondii RH 菌株鲎虫的抑制作用：结果：D. polychaetum 在体外具有宝贵的疗效，在接触后 30 分钟和 90 分钟的疗效优于嘧啶胺和磺胺嘧啶（p < 0.05）。多毛仙人掌精油对细胞株具有抗弓形虫活性（IC50：241.7 μg/mL）。用不同浓度的 D. 多毛象甲虫精油培养感染了 T. gondi 的 Vero 细胞后，它们的存活率以剂量依赖的方式下降：总之，多毛象甲虫提取物作为一种草药，可能是弓形虫病常规化疗的一种有价值的替代疗法。

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