The role of nitric oxide on the antiarrhythmic effects of ketamine/xylazine in a rat model of acute cardiac ischemia-reperfusion

IF 2.1 Q3 PHYSIOLOGY
Alireza Imani , Sulail Fatima Rajani , Kamran Rakhshan , Mahdieh Faghihi , Masoumeh Nemati , Tanaz Parsazadegan
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引用次数: 2

Abstract

The prevalence of ventricular arrhythmias during general anesthesia is about 70%. In experimental studies on the antiarrhythmic effects of different agents, using anesthetic drugs that do not have any protective properties are preferable. The present study was conducted to investigate molecular mechanisms involved in the antiarrhythmic effects of ketamine/xylazine (K/X).

Sixty male rats were assigned to eight groups: K/X, L -NAME (25–35 mg/kg) with thiopental (TP), L-NAME (25–35 mg/kg) with ketamine/xylazine, L arginine (100 mg/kg) with thiopental, L-arginine (100 mg/kg) with ketamine/xylazine. After anesthetic induction using TP or K/X, the animals were subjected to 30 min of ischemia. Hemodynamic parameters, ventricular arrhythmias during ischemia, the incidence of ventricular tachycardia (VT), and ventricular fibrillation (VF) were measured. Additionally, in order to assess nitrite/nitrate ratio and LDH after ischemia, serum samples were collected and used.

Our results showed that in the K/X group, the number of VT and VF, duration of VT (p = 0.006), the severity of arrhythmias (p = 0.0179). There was no VF incidence in this group.

These protective effects were faded by administration of L-NAME with K/X. The combination of L- Arginine in the TP group decreased the number and duration of VT (p < 0.001, p = 0.0013) with no incidence of VF in comparison with TP. L-arginine with K/X groups increased the number and duration of VT (p < 0.0001, p < 0.001) compared to K/X and VF was seen (100%). However, there was no significant difference between TP and K/X groups in terms of this nitrite/nitrate ratio. These findings suggest that the antiarrhythmic effects of ketamine/xylazine might be partially relative to the nitric oxide synthesis pathway.

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一氧化氮在大鼠急性心肌缺血再灌注模型中对氯胺酮/噻嗪抗心律失常作用的影响
全身麻醉时室性心律失常的发生率约为70%。在不同药物抗心律失常作用的实验研究中,使用不具有任何保护特性的麻醉药物是可取的。本研究旨在探讨氯胺酮/噻嗪(K/X)抗心律失常的分子机制。将60只雄性大鼠分为8组:K/X, L-NAME (25-35 mg/kg)与硫喷妥钠(TP), L-NAME (25-35 mg/kg)与氯胺酮/噻嗪,L精氨酸(100 mg/kg)与硫喷妥钠,L-精氨酸(100 mg/kg)与氯胺酮/噻嗪。TP或K/X麻醉诱导后,大鼠缺血30min。测量血流动力学参数、缺血时室性心律失常、室性心动过速(VT)发生率、室性颤动(VF)发生率。此外,为了评估缺血后亚硝酸盐/硝酸盐比率和LDH,采集血清样本并使用。结果显示,在K/X组中,VT数、VF数、VT持续时间(p = 0.006)、心律失常严重程度(p = 0.0179)的差异有统计学意义。本组无VF发病。这些保护作用在L-NAME与K/X联合使用后逐渐消失。TP组联合应用L-精氨酸可减少VT次数和持续时间(p <0.001, p = 0.0013),与TP相比无VF发生率。l -精氨酸加K/X组使VT次数和持续时间增加(p <0.0001, p <0.001),与K/X和VF相比(100%)。而TP组与K/X组之间的亚硝酸盐/硝酸盐比值差异不显著。这些发现提示氯胺酮/噻嗪的抗心律失常作用可能部分与一氧化氮合成途径有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.20
自引率
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审稿时长
62 days
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