Relationship between Human Papillomavirus Prevalence and DNA Damage in Cervical Cancer Population in Gansu Province, China.

IF 3.2 4区医学 Q3 VIROLOGY

Intervirology Pub Date : 2022-01-01 Epub Date: 2022-07-12 DOI:10.1159/000525975

Zhong Guo, Tianyuan Zhu, Yunsong Zhou, Tianbin Si, Qiang Wang, Hongmei Qu, Chenjing Wang, Jin Zhao

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引用次数: 1

Abstract

Objectives: The aim of this study was to assess the possible reason of the high incidence and mortality of cervical cancer in Longnan, China.

Materials and methods: 147 and 124 invasive squamous-cell carcinoma (SCC) samples from Longnan and different cities and districts of Gansu province were collected in the present study. All the samples were obtained from patients who underwent biopsies with colposcopy or advanced operations and were evaluated by experienced pathologists. HPV genotypes were examined with a validated HPV subtypes kit. The prevalence of HPV infection in SCC patients of China was analyzed by evidence-based medicine in the published literature. The markers of DNA damage response (DDR) - ATMpSer1981, H2AXp Ser139 (γH2AX), Chk2pThr68, and p53 - were analyzed by immunohistochemistry.

Results: HPV positivity, high-risk and multiple HPV positivity, and HPV58 infection were significantly higher in Longnan. Our results show that the prevalence of HPV infection in SCC patients of Longnan are consistent with the HPV prevalence in China. ATM, γH2AX, and p53 expressions in total and HPV+ samples were also higher in Longnan.

Conclusions: HPV-related DDR activation may be one reason for the high incidence and mortality of Longnan cervical cancer.

Abstract Image

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甘肃省宫颈癌人群中人乳头瘤病毒流行与DNA损伤的关系

目的:本研究的目的是评估中国陇南地区宫颈癌高发病率和高死亡率的可能原因。材料与方法:收集陇南及甘肃省不同市区147例、124例浸润性鳞状细胞癌(SCC)标本。所有样本均来自于接受阴道镜活检或晚期手术的患者，并由经验丰富的病理学家进行评估。用验证的HPV亚型试剂盒检测HPV基因型。采用循证医学方法分析中国SCC患者HPV感染的流行情况。免疫组织化学分析DNA损伤反应(DDR)标志物ATMpSer1981、H2AXp Ser139 (γH2AX)、Chk2pThr68和p53 -。结果:陇南地区HPV阳性、高危及多发HPV阳性、HPV58感染明显增高。我们的研究结果表明，陇南SCC患者的HPV感染患病率与中国的HPV患病率一致。在陇南地区，ATM、γ - h2ax和p53在总和HPV+样品中的表达也较高。结论:hpv相关的DDR激活可能是陇南宫颈癌高发病率和高死亡率的原因之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.