Exploring the use of a Ruthenium complex incorporated into a methacrylate-based dental material for antimicrobial photodynamic therapy.

IF 3.1 4区 医学 Q2 BIOPHYSICS
Adriana Pigozzo Manso, Maria Luísa Leite, Patricia Comeau, Claudia Dietrich, Sahand Ghaffari, Dirk Lange, Neil Branda
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引用次数: 5

Abstract

Objectives: To evaluate the effects of a blue light photosensitizer (PS), Ruthenium II complex (Ru), on the chemical, physical, mechanical, and antimicrobial properties of experimental dental resin blends.

Methods: The experimental resin (BisEMA, TEEGDMA, HPMA, ethanol, and photoinitiator) was loaded with Ru at 0.00%, 0.07%, 0.14%, 0.28%, 0.56%, 1.12%, 1.2%, 1.5%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% w/w. Samples were evaluated for the degree of conversion (DC) after 30 and 60 s curing-time (n = 6). Selected formulations (0.00%, 0.28%, 0.56%, 1.12%) were further tested for shear bond strength (SBS) (n = 15); flexural strength (FS) (n = 12); and antimicrobial properties (CFUs), in dark and light conditions. These latter tests were performed on specimens stored for 24-h or 2-month in 37°C water. Water sorption (WS) and solubility (SL) tests were also performed (n = 12). Data were analyzed either by a one- or two-factor general linear model (α = 0.05).

Results: Overall, Ru concentration above 1.2% resulted in reduced DC. In SBS results, only the 1.12%Ru resin blend samples had statistically lower values compared to the 0.00%Ru resin blend at 24-h storage (p = 0.004). In addition, no differences in SBS were detected among the experimental groups after 2-month storage in water. Meanwhile, FS increased for all experimental groups under similar aging conditions (p < 0.001). Antimicrobial properties were improved upon inclusion of Ru and application of light (p < 0.001 for both) at 24-h and 2-month storage. Lastly, no detectable changes in WS or SL were observed for the Ru-added resins compared to the 0.00%Ru resin blend. However, the 0.28% Ru blend presented significantly higher WS compared to the 0.56% Ru blend (p = 0.007).

Conclusions: Stable SBS, improved FS, and sustained antimicrobial properties after aging gives significant credence to our approach of adding the Ruthenium II complex into dental adhesive resin blends intended for an aPDT approach.

探索将钌配合物结合到甲基丙烯酸酯基牙科材料中用于抗菌光动力治疗。
目的:评价蓝光光敏剂钌II配合物(Ru)对实验性牙科树脂共混物的化学、物理、机械和抗菌性能的影响。方法:实验树脂(BisEMA、TEEGDMA、HPMA、乙醇和光引发剂)以0.00%、0.07%、0.14%、0.28%、0.56%、1.12%、1.2%、1.5%、2%、3%、4%、5%、6%、7%、8%、9%或10% w/w负载Ru。在30和60 s固化时间(n = 6)后,评估样品的转化程度(DC)。选取配方(0.00%、0.28%、0.56%、1.12%)进行剪切粘结强度(SBS)测试(n = 15);抗弯强度(FS) (n = 12);和抗菌性能(CFUs),在黑暗和光明条件下。后一项试验是在37°C水中保存24小时或2个月的标本上进行的。还进行了吸水性(WS)和溶解度(SL)试验(n = 12)。数据采用单因素或双因素一般线性模型进行分析(α = 0.05)。结果:总体而言,Ru浓度高于1.2%导致DC降低。在SBS结果中,只有1.12%Ru树脂共混样品在24小时的存储时比0.00%Ru树脂共混样品有统计学意义上的低值(p = 0.004)。另外,在水中贮藏2个月后,各试验组间的SBS无显著差异。同时,在相同老化条件下,各实验组FS均升高(p p p = 0.007)。结论:稳定的SBS,改善的FS和老化后持续的抗菌性能,为我们将钌II配合物添加到用于aPDT方法的牙科粘合剂树脂混合物中提供了重要的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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