Extracellular vesicles containing the I-BAR protein IRSp53 are released from the cell plasma membrane in an Arp2/3 dependent manner

IF 2.4 4区生物学 Q4 CELL BIOLOGY

Biology of the Cell Pub Date : 2022-07-17 DOI:10.1111/boc.202100095

Aurore de Poret, Rayane Dibsy, Peggy Merida, Alice Trausch, Kaushik Inamdar, Delphine Muriaux

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引用次数: 5

Abstract

Backgroud

Extracellular vesicles (EVs) are nanometric membrane vesicles produced by cells and involved in cell–cell communication. EV formation can occur in endosomal compartments whose budding depends on the ESCRT machinery (i.e., exosomes), or at the cell plasma membrane (i.e., EVs or microvesicles). How these EVs bud from the cell plasma membrane is not completely understood. Membrane curvatures of the plasma membrane toward the exterior are often generated by I-BAR domain proteins. I-BAR proteins are cytosolic proteins that when activated bind to the cell plasma membrane and are involved in protrusion formation including filopodia and lamellipodia. These proteins contain a conserved I-BAR domain that senses curvature and induces negative membrane curvatures at the plasma membrane. I-BAR proteins, such as IRSp53, also interact with actin co-factors to favor membrane protrusions.

Results

Here, we explore whether the I-BAR protein IRSp53 is sorting with EVs and if ectopic GFP-tagged I-BAR proteins, such as IRSp53-GFP, as well as related IRTKS-GFP or Pinkbar proteins, can be found in these EVs originated from the cell plasma membrane. We found that a subpopulation of these I-BAR EVs, which are negative for the CD81 exosomal biomarker, are produced from the cell plasma membrane in a TSG101-independent manner but in an Arp2/3-dependent manner.

Conclusions

Our results thus reveal that IRSp53 containing EVs represent a subset of plasma membrane EVs whose production depends on branched actin.

Significance

IRSp53 belongs to the I-BAR family proteins involved in curving cell membranes through a link with cortical actin. In that perspective, IRSp53 was shown to help membrane curvature of HIV-1 particles and, here, to be part of the budding process of a sub-population of EVs through its link with Arp2/3. IRSp53 is consequently a biomarker of these EVs of the cell plasma membrane.

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含有I-BAR蛋白IRSp53的细胞外囊泡以Arp2/3依赖的方式从细胞膜释放

细胞外囊泡(EVs)是细胞产生并参与细胞间通讯的纳米膜囊泡。囊泡的形成可以发生在内体腔室，其出芽取决于ESCRT机制(即外泌体)，也可以发生在细胞膜(即囊泡或微泡)。这些电动汽车是如何从细胞质膜发芽的还不完全清楚。质膜向外的膜曲率通常是由I-BAR结构域蛋白产生的。I-BAR蛋白是一种胞质蛋白，当被激活时，它与细胞膜结合，并参与包括丝状足和板足在内的突起形成。这些蛋白含有一个保守的I-BAR结构域，该结构域感知曲率并在质膜处诱导负膜曲率。I-BAR蛋白，如IRSp53，也与肌动蛋白辅助因子相互作用，有利于膜突出。本研究探讨了I-BAR蛋白IRSp53是否与EVs分选，以及是否可以在这些源自细胞膜的EVs中发现异位gfp标记的I-BAR蛋白，如IRSp53- gfp，以及相关的IRTKS-GFP或Pinkbar蛋白。我们发现这些I-BAR ev的一个亚群，CD81外泌体生物标志物阴性，以tsg101独立的方式从细胞膜产生，但以arp2 /3依赖的方式产生。因此，我们的研究结果表明，含有IRSp53的ev是质膜ev的一个子集，其产生依赖于支链肌动蛋白。IRSp53属于I-BAR家族蛋白，通过与皮质肌动蛋白的联系参与细胞膜弯曲。从这个角度来看，IRSp53被证明有助于HIV-1颗粒的膜曲率，并且通过其与Arp2/3的联系，成为ev亚群出芽过程的一部分。因此，IRSp53是细胞膜上这些ev的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biology of the Cell 生物-细胞生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal publishes original research articles and reviews on all aspects of cellular, molecular and structural biology, developmental biology, cell physiology and evolution. It will publish articles or reviews contributing to the understanding of the elementary biochemical and biophysical principles of live matter organization from the molecular, cellular and tissues scales and organisms. This includes contributions directed towards understanding biochemical and biophysical mechanisms, structure-function relationships with respect to basic cell and tissue functions, development, development/evolution relationship, morphogenesis, stem cell biology, cell biology of disease, plant cell biology, as well as contributions directed toward understanding integrated processes at the organelles, cell and tissue levels. Contributions using approaches such as high resolution imaging, live imaging, quantitative cell biology and integrated biology; as well as those using innovative genetic and epigenetic technologies, ex-vivo tissue engineering, cellular, tissue and integrated functional analysis, and quantitative biology and modeling to demonstrate original biological principles are encouraged.