A Koro, I Elezaj, S Hadžiabdić, A Alić, E Rešidbegović
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引用次数: 1
Abstract
Background: Infected poultry is one of the most important reservoirs of Salmonella.
Aims: The investigation presented here was conducted to examine the occurrence of Salmonella in fecal samples among selected flocks of backyard poultry in Bosnia and Herzegovina (B&H).
Methods: Isolation and identification of Salmonella was performed in accordance with BAS EN ISO 6579/AMD 1:2007. When genus Salmonella was confirmed, the determination of the antigenic formula of Salmonella isolates was performed in accordance with BAS CEN ISO/TR 6579-3:2015. After that, Salmonella serotypes were subjected to antibiotic susceptibility testing using EUVSEC sensititre microtiter plates impregnated with different concentrations of antibiotics. At the end, real-time PCR was used to detect extended spectrum β-lactamases (ESBL) and carbapeneamase encoding genes (blaTEM, blaSHV, blaCTX-M, blaCMY, blaKPC, blaNDM, blaOXA-48, blaVIM and blaGES).
Results: Salmonella spp. was detected in pooled feces from four backyards, housed by chickens only. Three isolates were confirmed by slide agglutination as serotype Enteritidis and one as serotype Typhimurium. Antibiotic susceptibility testing by microdilution did not reveal phenotypical resistance among these four isolates. Real-time PCR used to detect ESBL and carbapeneamase encoding genes revealed the blaTEM gene in one S. Enteritidis isolate.
Conclusion: Data presented in this study provide further evidence on the circulation of different Salmonella serotypes in backyard poultry in B&H. These findings emphasize the potential role of backyard poultry in the epidemiology of salmonellosis and the risks it poses for keepers, consumers, and general public health.
期刊介绍:
The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.