Different effects of magnetic field on drug activity in human uterine sarcoma cell lines MES-SA and MES-SA/Dx5.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-07-03 Epub Date: 2022-07-05 DOI:10.1080/15368378.2022.2095645

Reo Shibaki, Makiko Kakikawa

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引用次数: 0

Abstract

Previous studies reported that combined effect of magnetic field (MF) on cytotoxic drugs in human cancer cells. We focused on the effects of 60 Hz MF on drug activity in human uterine sarcoma MES-SA and drug-resistant variant MES-SA/Dx5 cells that overexpressed the membrane protein MDR1(P-glycoprotein), a drug efflux transporter for doxorubicin, daunorubicin, and etoposide, but not cisplatin. The cisplatin with MF caused 60% decrease in cell viability when compared with no MF treatment, cisplatin alone in MES-SA cells. Even in MES-SA/Dx5 cells, MF exposure equally enhanced cisplatin activity. Then, MF enhanced doxorubicin and daunorubicin activity in MES-SA cells and caused 60% decrease in the cell viability compared with these drugs only but had less effect on these drugs in MES-SA/Dx5 cells. Etoposide activity was unaffected by MF exposure in both cell lines, although etoposide is a MDR1 substrate as with doxorubicin and daunorubicin. Thus, MF had no direct impact on MDR1 in the cell membrane. However, the differences in doxorubicin and daunorubicin activity between MES-SA and MES-SA/Dx5 data revealed that the presence of MDR1 in abundance prevented the enhancing effects of MF on doxorubicin and daunorubicin activity. These results suggested that MF may act in the opposite direction of MDR1, affect the drug influx transporters for doxorubicin and daunorubicin, and facilitate anticancer drug uptake into the cells.

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磁场对人子宫肉瘤细胞系MES-SA和MES-SA/Dx5药物活性的不同影响

以往的研究报道了磁场(MF)对人癌细胞细胞毒性药物的联合作用。我们重点研究了60hz MF对人子宫肉瘤MES-SA和耐药变异MES-SA/Dx5细胞药物活性的影响，这些细胞过表达膜蛋白MDR1(p糖蛋白)，这是一种药物外排转运体，用于阿霉素、柔红霉素和依托泊苷，但不表达顺铂。在MES-SA细胞中，顺铂加MF组与不加MF组相比，细胞活力降低60%。即使在MES-SA/Dx5细胞中，MF暴露也同样增强了顺铂活性。然后，MF增强了MES-SA细胞中阿霉素和柔红霉素的活性，与单独使用这些药物相比，使细胞活力降低60%，但对MES-SA/Dx5细胞中这些药物的影响较小。在两种细胞系中，依托泊苷的活性不受MF暴露的影响，尽管依托泊苷与阿霉素和柔红霉素一样是MDR1底物。因此，MF对细胞膜中的MDR1无直接影响。然而，MES-SA和MES-SA/Dx5数据之间的多柔比星和柔红霉素活性差异表明，MDR1的大量存在阻止了MF对多柔比星和柔红霉素活性的增强作用。这些结果表明，MF可能与MDR1的作用方向相反，影响阿霉素和柔红霉素的药物内流转运体，促进抗癌药物进入细胞。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.