Profiling ATM regulated genes in Drosophila at physiological condition and after ionizing radiation.

IF 2.7 3区生物学

Hereditas Pub Date : 2022-10-21 DOI:10.1186/s41065-022-00254-9

Jun Liu, Tianyu Jin, Lanxi Ran, Ze Zhao, Rui Zhu, Gangcai Xie, Xiaolin Bi

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引用次数: 0

Abstract

Background: ATM (ataxia-telangiectasia mutated) protein kinase is highly conserved in metazoan, and plays a critical role at DNA damage response, oxidative stress, metabolic stress, immunity, RNA biogenesis etc. Systemic profiling of ATM regulated genes, including protein-coding genes, miRNAs, and long non-coding RNAs, will greatly improve our understanding of ATM functions and its regulation. RESULTS: 1) differentially expressed protein-coding genes, miRNAs, and long non-coding RNAs in atm mutated flies were identified at physiological condition and after X-ray irradiation. 2) functions of differentially expressed genes in atm mutated flies, regardless of protein-coding genes or non-coding RNAs, are closely related with metabolic process, immune response, DNA damage response or oxidative stress. 3) these phenomena are persistent after irradiation. 4) there is a cross-talk regulation towards miRNAs by ATM, E2f1, and p53 during development and after irradiation. 5) knock-out flies or knock-down flies of most irradiation-induced miRNAs were sensitive to ionizing radiation.

Conclusions: We provide a valuable resource of protein-coding genes, miRNAs, and long non-coding RNAs, for understanding ATM functions and regulations. Our work provides the new evidence of inter-dependence among ATM-E2F1-p53 for the regulation of miRNAs.

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ATM在果蝇生理状态和电离辐射后调控基因的分析。

背景:ATM (ataxia-毛细血管扩张突变)蛋白激酶在后生动物中高度保守，在DNA损伤应答、氧化应激、代谢应激、免疫、RNA生物发生等方面发挥重要作用。ATM调控基因的系统分析，包括蛋白质编码基因、mirna和长链非编码rna，将极大地提高我们对ATM功能及其调控的理解。结果:1)在生理状态和x射线照射后，鉴定了atm突变果蝇的差异表达蛋白编码基因、mirna和长链非编码rna。2)在atm突变果蝇中差异表达基因的功能，无论是蛋白质编码基因还是非编码rna，都与代谢过程、免疫反应、DNA损伤反应或氧化应激密切相关。3)辐照后这些现象持续存在。4)在发育过程中和辐照后，ATM、E2f1和p53对mirna有串扰调控。5)大多数辐照诱导的mirna敲除蝇或敲除蝇对电离辐射敏感。结论:我们为了解ATM的功能和调控提供了宝贵的蛋白质编码基因、mirna和长链非编码rna资源。我们的工作提供了新的证据，证明ATM-E2F1-p53之间相互依赖，调控mirna。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.