Human herpes virus type-6 is associated with central nervous system infections in children in Sudan.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-09-22 eCollection Date: 2022-01-01 DOI:10.4102/ajlm.v11i1.1718

Nada A Abdelrahim, Nahla Mohamed, Magnus Evander, Clas Ahlm, Imad M Fadl-Elmula

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引用次数: 3

Abstract

Background: Human herpes virus type-6 (HHV-6) is increasingly recognised as a febrile agent in children. However, less is known in sub-Saharan African countries, including Sudan.

Objective: We investigated the involvement of HHV-6 in paediatric central nervous system (CNS) infections in Khartoum, Sudan.

Methods: Febrile patients aged up to 15 years with suspected CNS infections at Omdurman Hospital for Children from 01 December 2009 to 01 August 2010 were included. Viral DNA was extracted from leftover cerebrospinal fluid (CSF) specimens and quantitatively amplified by real-time polymerase chain reaction (PCR) at Umeå University in Sweden.

Results: Of 503 CSF specimens, 13 (2.6%) were positive for HHV-6 (33.0% [13/40 of cases with proven infectious meningitis]). The median thermal cycle threshold for all HHV-6-positive specimens was 38 (range: 31.9-40.8). The median number of virus copies was 281.3/PCR run (1 × 10⁵ copies/mL CSF; range: 30-44 × 10³ copies/PCR run [12 × 10³ - 18 × 10⁶ copies/mL CSF]). All positive patients presented with fever and vomiting; 86.0% had seizures. The male-to-female ratio was 1:1; 50.0% were toddlers, 42.0% infants and 8.0% teenagers. Most (83.0%) were admitted in the dry season and 17.0% in the rainy season. Cerebrospinal fluid leukocytosis was seen in 33.0%, CSF glucose levels were normal in 86.0% and low in 14.0%, and CSF protein levels were low in 14.0% and high in 43.0%.

Conclusion: Among children in Sudan with CNS infections, HHV-6 is common. Studies on the existence and spread of HHV-6 chromosomal integration in this population are needed.

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6型人类疱疹病毒与苏丹儿童中枢神经系统感染有关。

背景:人类疱疹病毒6型(HHV-6)越来越被认为是儿童发热因子。然而，在包括苏丹在内的撒哈拉以南非洲国家，人们对这种情况知之甚少。目的:我们调查HHV-6在苏丹喀土穆儿童中枢神经系统(CNS)感染中的作用。方法:选取2009年12月1日至2010年8月1日在恩图曼儿童医院就诊的年龄不超过15岁的疑似中枢神经系统感染发热患者。从剩余的脑脊液(CSF)标本中提取病毒DNA，并在瑞典乌梅夫大学通过实时聚合酶链反应(PCR)进行定量扩增。结果:503份脑脊液标本中，13份(2.6%)HHV-6阳性(33.0%[13/40确诊感染性脑膜炎病例])。所有hhv -6阳性标本的中位热循环阈值为38(范围:31.9-40.8)。病毒拷贝数中位数为281.3个/PCR运行(1 × 105个拷贝/mL CSF;范围:30-44 × 103 copies/mL PCR [12 × 103 - 18 × 106 copies/mL CSF])。所有阳性患者均表现为发热和呕吐;86.0%有癫痫发作。男女比例为1:1;50.0%为幼儿，42.0%为婴幼儿，8.0%为青少年。旱季接待最多(83.0%)，雨季接待最多(17.0%)。脑脊液白细胞增多33.0%，脑脊液葡萄糖水平正常86.0%，低14.0%，脑脊液蛋白水平低14.0%，高43.0%。结论:在苏丹患有中枢神经系统感染的儿童中，HHV-6是常见的。需要对该人群中HHV-6染色体整合的存在和传播进行研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.