Lateral flow assay for rapid serodiagnosis of bovine leptospirosis.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2022-01-01 DOI:10.22099/IJVR.2021.41151.5974

K Senthilkumar, G Ravikumar

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引用次数: 0

Abstract

Background: Leptospirosis is considered to be an economically important disease in bovine. The disease burden is not appropriately monitored due to cumbersome serological tests that could be performed only in established laboratories. This warrants the development of a field level rapid diagnostic test.

Aims: The study aimed to develop a lateral flow assay (LFA)-based pen-side diagnostic test to detect antibodies to Leptospira.

Methods: LFA strip was prepared with the heat extracted antigen from L. interrogans serovar Pomona. To assess the performance of the developed LFA, a total of 300 bovine serum samples with their clinical histories were used and the initial screening for Leptospira antibodies was performed by the standard microscopic agglutination test (MAT). The sensitivity, specificity, and agreement (kappa value) were calculated between developed LFA and MAT. The stability of LFA was evaluated on days 30, 60, 90, and 120.

Results: Out of 300 samples tested, 225 were positive, and 75 were negative on MAT and 208 were positive, and 92 were negative on LFA. The developed LFA had a sensitivity of 90.7% and a specificity of 94.7%. The results of the assay were substantially in agreement with MAT, with a kappa value of 0.79. The LFA strips were stable for 120 days at 4°C.

Conclusion: A Lateral flow assay-based rapid pen-side test was developed and its utility to diagnose bovine leptospirosis was evaluated.

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快速诊断牛钩端螺旋体病的横向流动试验。

背景:钩端螺旋体病被认为是牛的一种重要的经济疾病。由于只能在已建立的实验室进行繁琐的血清学检测，疾病负担没有得到适当监测。这就要求开发一种现场水平的快速诊断测试。目的:本研究旨在建立一种基于横向流动试验(LFA)的笔侧诊断试验来检测钩端螺旋体抗体。方法:采用热提抗原法制备LFA条带。为了评估所开发的LFA的性能，共使用300份具有临床病史的牛血清样本，并通过标准显微镜凝集试验(MAT)对钩端螺旋体抗体进行初步筛选。计算成熟LFA和MAT之间的敏感性、特异性和一致性(kappa值)。在第30、60、90和120天评估LFA的稳定性。结果:300份样本中，阳性225份，MAT阴性75份，阳性208份，LFA阴性92份。LFA的敏感性为90.7%，特异性为94.7%。实验结果与MAT基本一致，kappa值为0.79。LFA条带在4°C下稳定120天。结论:建立了一种基于侧流法的快速笔侧检测方法，并评价了其在牛钩端螺旋体病诊断中的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.