Trichinella spiralis-derived extracellular vesicles induce a protective immunity against larval challenge in mice.

IF 2.7 4区医学 Q3 IMMUNOLOGY

Pathogens and disease Pub Date : 2022-11-12 DOI:10.1093/femspd/ftac040

Dalia S Ashour, Fatma M Kh Ibrahim, Amira M Elshamy, Hager S Zoghroban

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引用次数: 2

Abstract

Human trichinellosis is a serious disease with no effective treatment till now. Recently, the protective immunity induced by parasite-derived extracellular vesicles (EVs) are studied for some parasites such as Echinostoma caproni. The current study aimed to investigate the novel Trichinella spiralis-derived EVs as a potential vaccine candidate for the first time in a mouse model. Trichinella spiralis EVs were isolated and identified using transmission electron microscopy, gel electrophoresis, protein content measurements, and beads-based flow cytometry. Vaccination was done by subcutaneous injection of two doses of 3.5 μg T. spiralis-derived EVs. We observed a significant reduction in T. spiralis adult worm and muscle larval counts in mice immunized with T. spiralis-derived EVs (EVs-Ts group) and controlled inflammatory changes in the intestine and muscles. The EVs-Ts group showed a higher level of IFN- γ, whereas the IL-4 secretion was elevated more in the EVs group (EVs group) and showed a lower level after challenge with T. spiralis infection (EVs-Ts group). This implies a mixed Th1/Th2 immune response with obvious Th1 polarization. Moreover, elevation of serum T. spiralis-specific IgG was reported. In conclusion, this preliminary study provides T. spiralis EVs as a promising candidate for future development of anti-Trichinella vaccine.

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旋毛虫衍生的细胞外囊泡诱导小鼠对幼虫攻击的保护性免疫。

人类旋毛虫病是一种严重的疾病，至今尚无有效的治疗方法。近年来，人们研究了寄生虫源性细胞外囊泡(EVs)对卡普罗尼棘孔虫等寄生虫的保护性免疫作用。目前的研究旨在首次在小鼠模型中研究新型旋毛虫衍生的ev作为潜在的候选疫苗。采用透射电镜、凝胶电泳、蛋白含量测定和珠状流式细胞术分离鉴定旋毛虫EVs。通过皮下注射两剂3.5 μg螺旋体衍生的ev进行疫苗接种。我们观察到，接种螺旋体衍生EVs (EVs- ts组)的小鼠螺旋体成虫和肌肉幼虫数量显著减少，并控制了肠道和肌肉的炎症变化。EVs- ts组IFN- γ水平较高，而IL-4分泌在EVs组(EVs组)升高较多，而在螺旋体感染攻毒后(EVs- ts组)水平较低。这意味着Th1/Th2混合免疫反应具有明显的Th1极化。此外，血清螺旋体特异性IgG升高也有报道。综上所述，该初步研究为今后开发抗旋毛虫疫苗提供了一个有前景的候选株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathogens and disease IMMUNOLOGY-INFECTIOUS DISEASES

CiteScore

7.40

自引率

3.00%

发文量

期刊介绍： Pathogens and Disease publishes outstanding primary research on hypothesis- and discovery-driven studies on pathogens, host-pathogen interactions, host response to infection and their molecular and cellular correlates. It covers all pathogens – eukaryotes, prokaryotes, and viruses – and includes zoonotic pathogens and experimental translational applications.